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| Identifier |
000367455 |
| Title |
Χαρακτηρισμός νέων αυτοαντιγόνων του πυρήνα |
| Alternative Title |
Characterization of new nuclear autoantibodies |
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Author
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Τσαγγαρίδου, Έλενα
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Thesis advisor
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Θεοδωρόπουλος, Παναγιώτης
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Reviewer
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Καστανάς, Ηλίας
Κουρούμαλης, Ηλίας
Γραβάνης, Αχιλλέας
Σταθόπουλος, Ε.
Τζαρδή, Μαρία
Καμπά, Μ.
|
| Abstract |
Detection of autoantibodies giving nuclear rim pattern by
immunofluorescence (anti-nuclear envelope antibodies - ANEA) in sera from
patients with primary biliary cirrhosis (PBC) is a useful tool for the diagnosis
and prognosis of the disease. Differences in the prevalence of ANEA in PBC
sera so far reported have been attributed to the methodology used for the
detection as well as to ethnic/geographical variations. Therefore, we
evaluated the prevalence of ANEA in sera of Greek patients with PBC by
using methods widely used by clinical laboratories and a combination of
techniques and materials.
We screened 103 sera by immunoblotting on nuclear envelopes and
indirect immunofluorescence (IIF) using cells and purified nuclei. Reactivities
against specific autoantigens were assessed using purified proteins, ELISA,
immunoprecipitation and mass spectrometry.
We found higher prevalence of ANEA when sera were assayed by IIF
on purified nuclei or cultured cells (50%) compared to Hep2 commercially
available slides (15%). Anti-gp210 antibodies were identified in 22.3% and
33% of sera using ELISA for the C-terminal of gp210 or both ELISA and
immunoprecipitation, respectively. Immunoblotting on nuclear envelopes
revealed that immunoreactivity for the 210 kDa zone is related to antigp210
antibodies (p < 0.0001). Moreover, we found that sera had antibodies for
lamins A (6.8%), B (1%) and C (1%) and LBR (8.7%), whereas none at all had
detectable anti-p62 antibodies.
The prevalence of ANEA or anti-gp210 antibodies is under-estimated
in PBC sera which are analyzed by conventional commercially available IIF or
ELISA, respectively. Therefore, new substrates for IIF and ELISA should be
included by clinical laboratories in the analysis of ANEA in autoimmune sera.
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| Language |
Greek |
| Subject |
Autoantibodies |
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Detection of anti-nuclear autoantibodies |
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Enzyme-linked innunosorbent assay (ELISA) |
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GP210 |
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Identification of anti-nuclear autoantibodies |
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Immunobloting |
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Immunoprecipitation |
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Indirect immunofluorescence |
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LBR |
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Lamins |
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P62 |
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Primary biliary cirrhosis |
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Έμμεσος ανοσοφθορισμός |
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Ανίχνευση αυτοαντισωμάτων του πυρήνα |
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Ανοσοαποτύπωση |
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Ανοσοκατακρήμνιση |
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Αυτοαντισώματα |
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Λαμίνες |
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Μέθοδος ενζυμοσυνδετής ανοσοαπορροφητικής μέτρησης |
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Πρωτοπαθής χολική κίρρωση |
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Ταυτοποίηση αυτοαντισωμάτων πυρήνα |
| Issue date |
2010-07-20 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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| Views |
237 |
Χαρακτηρισμός νέων αυτοαντιγόνων του πυρήνα
