E-Locus - Institutional Repository of the University of Crete - Μελέτη των μεταβολών της έκφρασης γονιδίων που σχετίζονται με το μεταβολισμό και τη δράση χημειοθεραπευτικών στον πρωτοπαθή όγκο και στους επιχώριους και μεσοθωρακικούς διηθημένους λεμφαδένες σε χειρουργήσιμο μη μικροκυτταρικό καρκίνωμα του πνεύμονα (ΜΜΚΚΠ)

Home Μελέτη των μεταβολών της έκφρασης γονιδίων που σχετίζονται με το μεταβολισμό και τη δράση χημειοθεραπευτικών στον πρωτοπαθή όγκο και στους επιχώριους και μεσοθωρακικούς διηθημένους λεμφαδένες σε χειρουργήσιμο μη μικροκυτταρικό καρκίνωμα του πνεύμονα (ΜΜΚΚΠ)

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Identifier

000417852

Title

Μελέτη των μεταβολών της έκφρασης γονιδίων που σχετίζονται με το μεταβολισμό και τη δράση χημειοθεραπευτικών στον πρωτοπαθή όγκο και στους επιχώριους και μεσοθωρακικούς διηθημένους λεμφαδένες σε χειρουργήσιμο μη μικροκυτταρικό καρκίνωμα του πνεύμονα (ΜΜΚΚΠ)

Alternative Title

Association of BRCA1, ERCC1, RAP80, PKM2, RRM1, RRM2, TS, TSP1 and TXR1 m RNA expression levels between primary tumors and infiltrated regional lymph nodes in patients with resectable non‐ small cell lung cancer

Author

Τρυφωνίδης, Κωνσταντίνος

Thesis advisor

Σουγκλάκος, Ιωάννης
Γεωργούλιας, Βασίλειος

Reviewer

Σταθόπουλος, Ε.

Abstract

Differences in gene expression levels between the primary tumors (PTs) and matched regional lymph nodal metastases (LNs) in patients with totally excised non‐ small cell lung cancer (NSCLC) were explored. Microdissected formalin‐ fixed paraffin‐ embedded (FFPE) samples from (PT) and their matched infiltrated LNs, from 239 patients [183 (with matched PT and LNs samples)‐ case& 56 PT only samples‐ control cohorts ] were analyzed for BRCA1, ERCC1, RAP80, PKM2, RRM1, RRM2, TS, TSP1 and TXR1 mRNA expression by quantitative real‐ time polymerase‐ chain reaction (PCR). Moderately positive correlation between the expression of each gene in the PT and the matched LNs was observed. Concordance rates between the PT and the LNs were: BRCA1 (67.7%), ERCC1 (68.4%), PKM2 (63.4%), RAP80 (68.8%), RRM1 (70.9%), RRM2 (69%), TS (72.9%), TSP1 (69.8%), TXR1 (63.7%). Expression levels and their differences were correlated with Relapse‐ Free Survival (RFS) and Overall Survival (OS). High BRCA1 PT in patients with squamous histology was associated with increased OS (p= 0.036). High TSP1 PT levels were shown to be the only independent prognostic factor for OS and RFS (p= 0.023 and p=0.007). PKM2 low levels in both PT and matched LNs were associated with better OS irrespective of the underlying histology (p= 0.031). RRM1 discordant levels between PT and matched LNs were associated with worse OS in squamous tumors (p= 0.019) compared to patients with both low expression in PT and LN.TXR1 high levels in both PT and matched LNs were associated with better OS in patients with squamous tumors (p= 0.007).These findings indicate that there is different gene expression between PT and matched LNs which may affect the outcome in early NSCLC and therefore PT’s molecular biology should not be the sole determinant for prognostication.

Language

Greek

Subject

Adjuvant chemotherapy

Gene expression

Lymph nodes

NSClC

Primary tumor

Tumor heterogeneity

Επικουρική χημειοθεραπεία

Ετερογένεια

Issue date