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| Identifier |
uch.chemistry.msc//2005samolis |
| Title |
Φασματοσκοπική μελέτη μιας πρωτείνης του Chlorobium Teridum με σκέδαση ακτίνων laser |
| Alternative Title |
Spectroscopic study of a protein from Chlorobium Teridum with laser scattering |
| Creator |
Samolis, Panagiotis
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| Abstract |
A protein was isolated from the outer membrane of the photosynthetic bacterium Chlorobium Tepidum and was studied using the light scattering technique . The protein was solubilized with the aid of various surfactants which are called detergents . Various detergents of different chemical substance were used and therefore the reagents had different chemical properties such as hydrophilic / hydrophobic interactions or steric hindrance . Light scattering is a semi elastic process in which the scattered light has approximately the same wavelength with the incident light , but not exactly the same . The semi-elastic process of light scattering is subdivided in two basic categories . a) Static Light Scattering (SLS) . Static scattering measures the average value of intensity of scattered light during the time duration of the experiment . b) Dynamic Light Scattering (DLS) , The dynamic scattering measures the fluctuations that the scattered light intensity undergoes during the time duration of the experiment and records specifically the time moments when the maximum fluctuations occur . The goal of the experiments is to obtain some physicochemical parameters for the biological macromolecule . For instance the gyroscopic radius (Rg) is the distance between the mass center of the macromolecule with its outmost edge . The hydrodynamic radius is an equally useful parameter . With the supposition that every solute molecule is surrounded by many solvent molecules , a vesicle called blob is formed with a spherical shape and a radius called Rh .
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| Issue date |
2005-04-01 |
| Date available |
2007-03-05 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
431 |
Φασματοσκοπική μελέτη μιας πρωτείνης του Chlorobium Teridum με σκέδαση ακτίνων laser
