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Identifier

000429742

Title

Εffect of nerve growth factor and synthetic neurosteroids on the phenotype of adipocytes

Alternative Title

Επίδραση του αυξητικού παράγοντα των νεύρων και συνθετικών νευροστεροειδών στο φαινότυπο των λιποκυττάρων

Author

Δεικτάκης, Μιχαήλ

Thesis advisor

Δερμιτζάκη, Ειρήνη

Reviewer

Βενυχάκη, Μαρία
Χαραλαμπόπουλος, Ιωάννης
Μαργιωρής, Ανδρέας

Abstract

The adipose tissue is mainly responsible for energy homeostasis and secretes a variety of factors. Energy is stored by adipocytes in the form of lipids which are used in the event of energy demand. When caloric intake is greater than expenditure, adipocytes tend to become hypertrophic, which poses the threat of obesity and its co-morbidities. Cold exposure and exercise are some major positive regulators of metabolic function, contributing to the browning of white adipose tissue. Both the fact that neurotrophin receptors reside in the adipose tissue and the need for the development of a metabolically beneficial drug led us to test the effect of nerve growth factor (NGF) and a synthetic microneurotrophin devoid of steroid actions, BNN27, on the adipocyte function. Human white mature adipocytes and pre-adipocytes were isolated from tissue samples offered by volunteers undergoing surgery. In addition, when the aforementioned were not available, the 3T3L1 pre-adipocyte cell line was used. NGF increased mitochondrial respiration and glycolysis in 3T3L1 pre-adipocytes and reduced mitochondrial mass, ROS production and the number of intracellular lipids in human mature adipocytes whereas, BNN27 increased metabolic functions but had not any significant effect on the adipocyte lipids content. Overall, the above results indicate that these molecules may have a possible role on the regulation of the metabolic profile of adipocytes.

Language

Greek

Subject

Metabolic function

Αυξητικός παράγοντας των νεύρων

Λιποκύτταρα

Μεταβολική λειτουργία

Νευροστεροειδή

Φλεγμονώδης φαινότυπος

Issue date

2019-12-11

Collection