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| Identifier |
000403388 |
| Title |
The role of MS-PV GABAergic neurons and theta rhythm in the regulation of REM sleep |
| Alternative Title |
Ο ρόλος των MS-PV GABAεργικών νευρώνων και του ρυθμού θήτα στη ρύθμιση του ύπνου REM |
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Author
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Βολιτάκη, Εμμανουέλα
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Thesis advisor
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Korotkova, Tatiana
Ponomarenko, Alexey
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Reviewer
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Καραγωγέως, Δόμνα
Σιδηροπούλου, Κυριακή
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| Abstract |
Rapid-eye-movement (REM) is a distinct phase of sleep and the mechanisms responsible for its regulation remain to be clarified. Theta (5-10 Hz) is the dominant brain rhythm present in the EEG and in all hippocampal regions during REM sleep. The medial septum-diagonal band of Broca (MS-DBB) neurons are considered as the fundamental theta pacemakers. Crucial for pacing of theta are the MS parvalbumin-positive (PV) GABAergic cells which synapse onto interneurons in hippocampus CA1 and rhythmically release pyramidal cells from inhibition. In this pilot study, we examined the involvement of MS-PV GABAergic neurons and hippocampal theta oscillations in REM sleep regulation. To address this question, we combined in vivo electrophysiological recordings in behaving mice with optogenetic stimulation of the MS-PV GABAergic neuronal projections to hippocampus (HC) CA1 region during different vigilance states. Our results indicated that optogenetic stimulation of MS-hippocampal projections during REM sleep at theta 8 Hz frequency reduced the time intervals between consequent REM episodes by nearly 4 minutes compared to the equivalent control light experiments and disrupted the relationship between REM sleep episode duration and the preceding REM sleep interval, without disrupting vigilance state architecture in general. Moreover it was demonstrated that theta oscillation entrainment fidelity around transitions from non REM (NREM) sleep to active wakefulness (AW) was higher than around transitions from quiet wakefulness (QW) to NREM sleep. These preliminary results could indicate that MS-PV GABAergic neurons and the theta rhythm which they pace, are involved in the circuit that regulates REM sleep.
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| Language |
English |
| Subject |
Channelrhodopsin-2 |
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Hippocampus |
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Medical septum |
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Optogenetics |
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Διάφραγμα |
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Ιππόκαμπος |
| Issue date |
2016--12-13 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
267 |
The role of MS-PV GABAergic neurons and theta rhythm in the regulation of REM sleep
