| Abstract |
Breast cancer is the most frequent cancer in the female population and involvement of chromosomal alterations is often implicated in the development of cancer. The aim of our study was to assess loss of heterozygosity (LOH) in relation to clinical and pathological parameters, such as age, stage of the disease, tumor size, tumor grade, lymph node involvement, extensive intraductal component (EIC) and peritumoral angiolymphatic invasion (PALI). In detail, tumors, corresponding normal tissues and peripheral blood samples from 50 women with operable breast cancer, were analyzed by polymerase chain reaction (PCR) at 16 polymorphic DNA markers, on both the long and short arm of chromosome 1. We also studied the involvement of DNA mismatch repair genes in sporadic breast cancer, matched normal and tumoral DNA samples of the same patients were analyzed for LOH with 10 microsatellites at or linked to hMSH2 (2p16), hMLH1 (3p21-23) and hMSH3 (5q11-q13). There was a significant correlation between chromosomal region 1q21-23 (LOH 34%) and the presence of extensive intraductal component (EIC) and peritumoral angiolymphatic invasion (PALI) (p<0.01, p<0.04), both independent markers of local recurrence. The percentage of LOH 2p16, 3p21-23 and 5q11-13 regions was 12%, 16% and 8%. There was no statistical significant correlation with clinical and pathological parameters. Allelic loss in region 1q21-23 may be a value prognostic biological marker for the detection of local relapse in breast cancer, in combination with other histological and clinical parameters.
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Aνάλυση μικροδορυφορικού DNA σε νεοπλασίες του μαστού
