E-Locus - Institutional Repository of the University of Crete - Σύγκριση των εξελικτικών ρυθμών των γονιδίων που κωδικοποιούνται από μιτοχονδριακό και πυρηνικό dna και που τα προϊόντα τους συνθέτουν τα σύμπλοκα της οξειδωτικής φωσφορυλίωσης. Διερεύνηση της ύπαρξης θετικής επιλογής σε γονίδια των συμπλόκων της οξειδωτικής φωσφορυλίωσης σε εξελικτικές γραμμές της οικογένειας δροσοφιλίδων

Home Σύγκριση των εξελικτικών ρυθμών των γονιδίων που κωδικοποιούνται από μιτοχονδριακό και πυρηνικό dna και που τα προϊόντα τους συνθέτουν τα σύμπλοκα της οξειδωτικής φωσφορυλίωσης. Διερεύνηση της ύπαρξης θετικής επιλογής σε γονίδια των συμπλόκων της οξειδωτικής φωσφορυλίωσης σε εξελικτικές γραμμές της οικογένειας δροσοφιλίδων

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Identifier

000361053

Title

Σύγκριση των εξελικτικών ρυθμών των γονιδίων που κωδικοποιούνται από μιτοχονδριακό και πυρηνικό dna και που τα προϊόντα τους συνθέτουν τα σύμπλοκα της οξειδωτικής φωσφορυλίωσης. Διερεύνηση της ύπαρξης θετικής επιλογής σε γονίδια των συμπλόκων της οξειδωτικής φωσφορυλίωσης σε εξελικτικές γραμμές της οικογένειας δροσοφιλίδων

Alternative Title

Evolutionary behaviour analysis of the proteins that participate in the oxidative phosphorylation complexes, and are assembled by either nuclear of mitochondrial dna. A scan for positive selection in the oxidative phosphorylation complexes of ten drosophilidae species.

Author

Ζουμαδάκης, Χρήστος

Thesis advisor

Λαδουκάκης, Μανόλης

Abstract

The purpose of this study was: a) the analysis of the evolutionary behaviour of the proteins that participate in the oxidative phosphorylation pathway, and which are either assembled in the mitochondrion by mitochondrial DNA, or are assembled in the cell cytoplasm by nuclear DNA, and subsequently targeted to the mitochondrion. Also, it was researched whether certain characteristics of these proteins, such as their size, their number of protein-protein interactions, or the selection pressure that acts on them, have statistically significant correlations between each other. Results show that nuclear proteins that interact with mitochondrial ones in the respiratory pathway complexes, have similar evolutionary rates with the rest of the nuclear DNA proteins. These evolutionary rates are faster in animals than the respective mitochondrial rates, but slower in plants. However in order for the proteins from different genetic backgrounds (mitochondrial and nuclear) to co-interact in order to build the respiratory pathway complexes, natural selection plays an important role which seems to be stronger in mitochondrial proteins. Again, the opposite pattern can be observed in plants. In both types, positive correlation was found between their size and the number of protein interactions. In specific protein complexes, correlation between selection pressure, and either length, or number of interaction partners was shown. b) the application of specific maximum likelihood techniques, in order to quantify the selection pressure that acts in the oxidative phosphorylation genes of ten Drosophilidae species, as well as to compare these rates, among all the nodes of their phylogenetic tree. The results show that a number of genes have different selection pressures acting on them, in many of the tree nodes. Moreover, traces of positive selection were identified in a number of genes, with most of them detected in the D.pseudoobscura and D.persimilis species, after their divergence from their common ancestor.

Language

Greek

Subject

Drosophila

Mitochondrion

Δροσόφιλα

Μιτοχόνδριο

Issue date

2010-11-19

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Views

301

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