E-Locus - Institutional Repository of the University of Crete - Ανάλυση πατρότητας κι εκτίμηση γενετικών παραμέτρων σε διαφορετικά αναπτυξιακά στάδια στο είδος Argyrosomus regius

Home Ανάλυση πατρότητας κι εκτίμηση γενετικών παραμέτρων σε διαφορετικά αναπτυξιακά στάδια στο είδος Argyrosomus regius

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Identifier

000448658

Title

Ανάλυση πατρότητας κι εκτίμηση γενετικών παραμέτρων σε διαφορετικά αναπτυξιακά στάδια στο είδος Argyrosomus regius

Alternative Title

Parentage analysis and genetic parameter estimation at different developmental stages in meagre Argyrosomus regius

Author

Τασιούλη, Αικατερίνη Ε.

Thesis advisor

Τσιγγενόπουλος, Κωνσταντίνος

Reviewer

Χατζηπλής, Δημήτριος
Μανουσάκη, Τερέζα

Abstract

Meagre is a sciaenid species with high commercial interest for the aquaculture industry. Due to its recent history in culture conditions, the study of genetic parameters on economically important traits is limited. The aim of the present work was to investigate the family structure and parental contribution of thirteen meagre broodstock and 1,200 offspring from a mass spawning event, as well as to estimate genetic parameters for body weight and length in different ages. Parentage analysis was conducted with a multiplex of 12 microsatellite loci using an exclusion-based method. The expected assignment power of the multiplex used was simulated at a medium rate of 65.9%, but in fact achieved a higher assignment rate ~72%. The offspring distribution in families was unbalanced emerging from an unequal contribution of broodstock in both sexes, with almost half of the broodstock (7/13) presenting a dominant role. The offspring of the dominant broodstock formed six big families, which represented percentages ranging between 4-30%, while the rest of the families represented <2% each. Genetic parameter estimation was conducted using the Restricted Maximum Likelihood method, resulting in high estimated values of heritability for the studied traits, indicating the suitability of the studied traits to be selection objectives in a breeding program with a high expected genetic gain. At the same time the heritability estimates tended to increase with age. Furthermore, the high estimated genetic correlation between body weight and length suggests the possibility of selection using only one of the two traits, as selection criteria. Genetic correlation between weights at different ages presented lower estimated values. A major setback in the present study was the high fish mortality which led to limited data from the latter studied ages and resulted in high standard errors. Thus, the repetition of the study using a larger population is proposed in order to acquire more accurate estimates of traits of economic interest in meagre.

Language

Greek, English

Subject

Genetic parameters

Heritability

SSR

Γενετικές παραμέτροι

Κληρονομησιμότητα

Issue date

2022-07-29

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses