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Identifier

000451648

Title

Genomic assembly and bioinformatic analyses of devil firefish (Pterois miles) genome using Oxford Nanopore, Pacific Biosciences and Illumina sequencing technologies

Author

Κιτσούλης, Χρήστος

Thesis advisor

Μανουσάκη, Τερέζα
Ηλιόπουλος, Ιωάννης

Abstract

Devil firefish (Pterois miles), member of Scorpaenidae, is one of the most successful marine invaders, dominating around the world, that was rapidly spread into the Mediterranean Sea, through the Suez Canal, originating from the Red Sea. In this study we built and analyzed the first reference genome assembly of P. miles and explored its evolutionary background. Through genome analysis, we revealed the big amount of transposable elements present on the genome, while with phylogenomic analyses we constructed the first fish phylogeny which includes a member of genus Pterois, based on whole genome sequencing data. Furthermore, we identified six individual genes that encode the two subunits (three genes per subunit) of the toxins secreted from devil firefish’s spines and are responsible for the harmful touch, adding a major baseline for additional studies on the lionfish toxins origin and evolution. The high-quality and contiguity genome assembly built here provides a valuable resource for future studies about the species biology, the influence of transposable elements on the evolution of vertebrate genomes and fish toxins evolution.

Language

English

Subject

Gene duplication

Genome analysis

Phylogenomic analyses

Transposable elements

Ανάλυση γονιδιώματος

Γονιδιακός διπλασιασμός

Λεοντόψαρο

Μεταθετά στοιχεία

Τοξίνες