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Identifier 000451013
Title Μελέτη της επίπτωσης της μακροχρόνιας κρυοσυντήρησης στα αρχέγονα αιμοποιητικά κύτταρα
Alternative Title The effect of long term cryopreservation on hematopoietic stem cells
Author Μουτσάκη, Εμμανουέλα
Thesis advisor Στειακάκη, Ευτυχία
Reviewer Ποντίκογλου, Χαράλαμπος
Καλπαδάκη, Χριστίνα
Abstract The effect of long term cryopreservation on hematopoietic stem cells In transplantation, the hemopoietic stem cells are collected either from the bone marrow or the peripheral blood upon mobilization. Peripheral blood ultimately is the most preferable source of HSCs (approximately ⅔ of transplantations effectuated). The CD34 surface antigen, expressed by the HSCs is used in order to evaluate the quality and quantify the transplant. In order to achieve a successful engraftment safe and efficient storage of the cells is imperative. The transplant engraftment in the bone marrow niches is influenced by the CD34 number and clonogenic potential, the cryοpreservation the thawing technique and the storage conditions of the graft. Osmotic stress as well as intra- and extracellular ice formation in the process of cryopreservation might compromise the cell viability. Moreover, disturbance of the mitochondrial function, DNA damage, disruption of repair mechanisms and gene expression could result in cell death. The most important factors that determine the quality of cryopreservation appear to be the cooling rate and the type and concentration of the cryoprotectant applied. Data regarding the effects of cryopreservation on HSC viability and their clonogenic potential are limited and the results contradictory. Both loss of cell viability after a few months of storage, and successful transplantation several years after the cryopreservation are described. The aim of this study was to estimate the effects of cryopreservation in the viability and clonogenic potential of HSCs. The methods utilized were flow cytometry and methocult. In addition, trypan blue dye exclusion was applied. In conclusion maintenance in the viability of HSCs for 12-24 years of cryopreservation, their percentage in the graft and a high number of colonies for up to 19 years of cryopreservation were observed.
Language Greek
Subject Αποικίες
Βιωσιμότητα
Κυτταρομετρία ροής
Μεθυλοκυτταρίνη
Issue date 2022-07-29
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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