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| Title |
Βιοαποικοδήμηση της φαινόλης από το μικροφύκος Chlamydomonas reinhardtii κάτω υπό συνθήκες που επηρεάζουν τη φωτοσύνθεση |
| Alternative Title |
Biodegradation of phenol by the green microalga Chlamydomonas reinhardtii under conditions affecting photosynthesis |
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Author
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Κοκαράκης, Εμμανουήλ
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Thesis advisor
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Γανωτάκης, Δημήτρης
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| Abstract |
In the present work the biodegradation of phenol by the green microalga Chlamydomonas reinhardtii was studied. Biodegradation of phenol proved to be a dynamic bioenergetic process, significantly affected by the changes in culture conditions. More specifically the presence or absence of acetate in the culture medium, light intensity and oxygen availability was studied. Impact of phenol on culture growth and structure and function of the photosynthetic apparatus was studied as well. Finally biodegradation ability of some Chlamydomonas mutant strains was studied as well. Results showed that phenol is a growth inhibitive substrate, especially in concentrations over 2.00 mM. In addition higher initial concentrations of phenol led to higher biodegradation ability. In these concentrations the microalga was found able to biodegrade higher amounts of phenol. High concentrations of phenol led to evaluation of typical stress effects as they were expressed by the induced fluorescence (JIP-test) measurements and quantification of photosynthetic pigments content. In all cases, increased stress and inhibitory effects evaluated by high concentrations of phenol led to enhanced biodegradation. In addition biodegradation was higher in acetate- limited cultures. Biodegradation proved to be an aerobic and photoregulated process. Higher light intensity and oxygen availability led to increased biodegradation of phenol. Chlamydomonas reinhardtii mutant strains showed ability in biodegrading phenol as well. This study proves that biodegradation does not depend on selection of the appropriate microorganism but in the selection of appropriate culture conditions
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| Language |
Greek |
| Subject |
Bioenergetics |
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Stress |
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Βιοενεργητική |
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Καταπόνηση |
| Issue date |
2016-07-22 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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| Views |
315 |
Βιοαποικοδήμηση της φαινόλης από το μικροφύκος Chlamydomonas reinhardtii κάτω υπό συνθήκες που επηρεάζουν τη φωτοσύνθεση
