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Identifier |
000388620 |
Title |
Acoustic sensors for protein and cell binding |
Alternative Title |
Ακουστικοί αισθητήρες για μελέτη πρόσδεσης πρωτεϊνών και κυττάρων |
Author
|
Χρονάκη, Δήμητρα Ι.
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Thesis advisor
|
Γκιζελή, Ηλέκτρα
|
Reviewer
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Αθανασάκη, Ειρήνη
Χαλεπάκης, Γιώργος
|
Abstract |
In this study we gained a better insight on protein adsorption and cell adhesion processes using the acoustic biosensor Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). Specifically, real-time adsorption of three extracellular proteins was monitored on Au surfaces. The proteins tested were collagen, fibronectin and fibrinogen and their adsorption resulted in significant information regarding their orientation on Au. For results interpretation we used the “discrete model” theory, which is a molecular approach of the adsorbed layer rather than the usual film approach. Acoustic ratio measurements (ΔD/ΔF) were combined with Sauerbrey equation for monolayer coverage to predict orientation of adsorbed molecules. Furthermore, cell adhesion studies were performed for two cell lines: normal (Nthy) and malignant (ARO) thyroid cells. QCM-D technique was recruited to distinguish dynamic cell adhesion signatures of the two cell lines. QCM-D frequency and dissipation shifts were accompanied by optical microscopy images of the adhered cells. Binding isotherms proved the concentration-dependent nature of cell adhesion process and report a different binding capacity of Nthy and ARO on Au. Finally, cell adhesion kinetics revealed remarkable differences of two cell lines on the underlying substrate.
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Language |
English |
Subject |
Cell adhesion |
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Kinetics |
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Protein adsorption |
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Κινητική |
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Προσκόλληση κυττάρων |
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Προσρόφηση πρωτεϊνών |
Issue date |
2014-11-21 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Views |
326 |