E-Locus - Institutional Repository of the University of Crete - Χαρακτηρισμός του τύπου της ανοσολογικής απάντησης και μελέτη του Vβ ρεπερτορίου του υποδοχέα των Τ-λεμφοκυττάρων σε πάσχοντες από χρόνια ιδιοπαθή ουδετεροπενία

Home Χαρακτηρισμός του τύπου της ανοσολογικής απάντησης και μελέτη του Vβ ρεπερτορίου του υποδοχέα των Τ-λεμφοκυττάρων σε πάσχοντες από χρόνια ιδιοπαθή ουδετεροπενία

Results - Details

[Add to Basket]

Identifier

000369958

Title

Χαρακτηρισμός του τύπου της ανοσολογικής απάντησης και μελέτη του Vβ ρεπερτορίου του υποδοχέα των Τ-λεμφοκυττάρων σε πάσχοντες από χρόνια ιδιοπαθή ουδετεροπενία

Alternative Title

Clarification of the type of immune responce and T-cell receptor VB repertoire analysis in patients with chronic idiopathic neutropenia.

Author

Σπανουδάκης,Μιχαήλ Ι

Thesis advisor

Παπαδάκη, Ε

Reviewer

Μπούμπας, Δ.
Σουρβίνος, Γ

Abstract

Chronic idiopathic neutropenia is a bone marrow (BM) disorder characterised by increased apoptosis of the granulocytic progenitor cells and presence of activated T-lymhocytes in the peripheral blood (PB) and BM. We have investigated the pattern of PB and BM T-cell responses in CIN by analyzing the T-cell receptor -chain variable (V) segment diversity using flow-cytometry and molecular spectratyping. Compared to the controls (n=40), CIN patients (n=85) displayed a different pattern of Vβ usage in the PB CD3+, CD4+ and CD8+ cells. The frequency of Vβ skewing and the mean number of expanded Vβ subfamilies per subject were significantly higher in patients compared to controls in all cell subpopulations. Skewing was more profound within the CD8+ cells. The frequency of patients displaying V subfamily expansions was similar in BM and PB T-cell subsets however, the number of V expansions per patient was significantly higher in BM compared to PB cells. The spectratyping showed that the majority of patients displayed a skewed oligoclonal/monoclonal pattern within the PB and/or BM CD8+ cells and a polyclonal profile within the CD4+ cells. We concluded that aberrant, probably antigen-driven, T-cell expansions are invariably detected in CIN patients and may have a role in the disease pathogenesis.

Language

Greek

Subject

Bone marrow failure

Chronic idiopathic neutropenia

Hemic and lymphatic systems

T-cell receptor

Τ-λεμφοκυττάρων

Υποδοχέας Τ-λεμφοκυττάρων

Χρόνια ιδιοπαθής ουδετεροπενία

Issue date

2011-12-14