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| Identifier |
000397225 |
| Title |
Regulation of miR-155 in response to leptin in macrophages |
| Alternative Title |
Ρύθμιση των miR-155 αποκρινόμενα σε λεπτίνη στα μακροφάγα |
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Author
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Σοφιάδης, Κωνσταντίνος Χ.
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Thesis advisor
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Τσατσάνης, Χρήστος
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| Abstract |
The proper function of immune system requires availability of nutrients to promote a productive and robust response against infection. Adipose tissue is the main energy-producing factory that supplies the necessary nutrients to the diverse cellular responses. Thus all these diverse cellular responses require ways to communicate with adipose tissue to sufficiently regulate their activity and homeostasis. Immune system and adipose tissue possess the ability to communicate through soluble factors known as cytokines and adipokines.
Leptin is a very important adipokine highly and mainly expressed from the adipose tissue that controls the food intake and represent a marker of the energy availability in the organisms. Namely, leptin could serve as a sufficient mediator of the crosstalk between these systems, specifically modulating the dynamics of immune system in concordance with nutrients availability. On the other hand high circulating levels of this adipokine reminiscent to obesity might promote a chronic pro-inflammatory phenotype. In this study we attempt to elucidate some aspect of the inflammatory phenotype this hormone can confer in either unstimulated or LPS stimulated cells.
Furthermore, we investigate the role of mir155, an already established pro-inflammatory marker in response to leptin. We aim to discover the effect of leptin in the expression levels of this molecule and validate whether mir155 could mediate the signaling of leptin in unstimulated or LPS-stimulated macrophages.
We propose that leptin represent an adipokine that is able to induce a pro-inflammatory phenotype. We also that mir155 expression upon leptin treatment is also enhanced. On the other hand we do not demonstrate a clear association of mir155 in leptin signaling pathway in macrophages.
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| Language |
English |
| Subject |
Cytokine |
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Hormone |
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Inflammation |
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LPS |
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Κυτοκίνη |
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Λιποπολυσακχαρίτης |
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Ορμόνη |
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Φλεγμονή |
| Issue date |
2015-11-20 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
252 |
Regulation of miR-155 in response to leptin in macrophages
