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Home    Μελέτη της συμβολής των μεταγραφικών παραγόντων Sall1 και Nr0b1 στην αυτό-ανανέωση και πολυδυναμικότητα των εμβρυϊκών βλαστοκυττάρων  

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Identifier 000326701
Title Μελέτη της συμβολής των μεταγραφικών παραγόντων Sall1 και Nr0b1 στην αυτό-ανανέωση και πολυδυναμικότητα των εμβρυϊκών βλαστοκυττάρων
Author Ιωάννου, Μαρίλια
Thesis advisor Παπαματθαιάκης, Ιωσήφ
Κρετσόβαλη, Ανδρονίκη
Abstract During differentiation of mESCs, genes that are responsible for maintaining pluripotency such as Nanog, Oct4 and Sox2 are down regulated. Next to these factors we observed the suppression of two other genes, Sall1 and Nr0b1, which were not previously involved in the regulations of stem cell function. Sall1 is a Zn-finger transcriptional repressor that is essential for kidney development and has been linked to human Townes-Brocks syndrome. Nr0b1/Dax1 is an orphan nuclear receptor that is broadly expressed and has co-repressor activity. Both were found to be Nanog target genes in mESCs. The concurrent down regulation of Sall1 and Nr0b1 with the three core pluripotency factors during differentiation raises the possibility that there is a functional interconnection among them. Employing in vitro and in vivo assays we have shown that Sall1 and Nr0b1 interact with the three core pluripotency factors Nanog, Oct4 and Sox2. Moreover, using an in vitro/ GST pull-down assay, we showed that Sall1 can interact both with both Nanog and Sox2 at the same time. Furthermore in order to study which protein domains are responsible for this double interaction, we used only the N-terminal domain of Sall1 (aa 1-2024), and found that it can interact with Nanog but not with Sox2 or Oct4. Our results suggest that the protein complex of transcription factors required to maintain self-renewal and to preserve the undifferentiated state of ES cells may also include Sall1 and Nr0b1.
Language Greek
Issue date 2007-11-28
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Notes Διατμηματικό πρόγραμμα μεταπτυχιακών σπουδών:" Μοριακή Βιολογία - Βιοϊατρική"
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