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Identifier uch.biology.msc//2005tsanaka
Title Μελέτη πρόσδεσης της πρωτεΐνης IgG σε τροποποιημένη με νουτραβιδίνη επιφάνεια ακουστικής μικρισυσκευής
Author Τσανάκα, Χρυσούλα
Abstract The aim of this work is to use an acoustic wave sensor to detect the viscoelastic and mass changes on a solid / liquid interface when various concentrations of IgG and BSA are bound to the device surface. Devices which use shear-horizontal surface acoustic waves propagating in a wave guide configuration at 110MHz and 155MHz were, firstly, calibrated and characterized, and then used for the detection of specific binding and adsorption of biotinylated anti-human IgG (150kD) and biotinylated BSA (66kD) to neutravidin-modified surfaces. The detection of the biotinylated protein was in the concentration range of 9nM to 667nM. In order to calculate the mass of the protein bound to the neutravidin layer, same experiments took place on Qsense instrument, which also tracks mass changes on a piezoelectric surface. Results clearly indicate that the velocity of the acoustic wave (measured as phase) is sensitive to the mass of the bound analyte while the acoustic energy (measured as amplitude) is sensitive to the viscoelasticity of the bound layer, which is related to the shape of the molecules. Finally, experiments for the regeneration of the neutravidin layer took place.
Language Greek
Issue date 2005-11-28
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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