E-Locus - Institutional Repository of the University of Crete - Μελέτη της αυτοφαγίας στην ιδιοπαθή πνευμονική ίνωση (IPF) και στην αυτοάνοση πνευμονική ίνωση που σχετίζεται με την ρευματοειδή αρθρίτιδα (RA-UIP)

Home Μελέτη της αυτοφαγίας στην ιδιοπαθή πνευμονική ίνωση (IPF) και στην αυτοάνοση πνευμονική ίνωση που σχετίζεται με την ρευματοειδή αρθρίτιδα (RA-UIP)

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Identifier

000445617

Title

Μελέτη της αυτοφαγίας στην ιδιοπαθή πνευμονική ίνωση (IPF) και στην αυτοάνοση πνευμονική ίνωση που σχετίζεται με την ρευματοειδή αρθρίτιδα (RA-UIP)

Alternative Title

Study of autophagy in IPF idiopathic pulmonary fibrosis and in RA-UIP reumatic arthritis connected with autoimmune pulmonary fibrosis

Author

Σαραντουλάκη, Στυλιανή

Thesis advisor

Αντωνίου, Κατερίνα

Reviewer

Σουρβίνος, Γεώργιος
Τσατσάνης, Χρήστος

Abstract

Idiopathic pulmonary fibrosis is a chronic and irreversible interstitial lung disease with poor prognosis and limited treatment options. Rheumatoid arthritis is a chronic inflammatory autoimmune disease that is often complicated by the development of interstitial lung disease, leading to high mortality and morbidity. Autophagy is a process that regulates the turnover of subcellular components and damaged organelles and represents an important cellular homeostatic mechanism. Given that recent evidence suggests a role of autophagy in the development of IPF, the aim of this study is to investigate the mRNA levels of molecules – markers of the autophagic process in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis compared to patients with rheumatoid arthritis who experience lung involvement via RT-qPCR. A significant increase in BECLIN1 was observed in patients with RA-ILD compared to those with IPF. On the other hand, despite the lower expression of p62 in some patients with IPF, no significant association of the concentration of the p62 and Beclin1 proteins was observed between patients with IPF and healthy controls. In other autophagy responsible genes examined, such as ULK1, BNIP3 and p62, no differences in mRNA expression levels were observed between RA-ILD and IPF patients. For patients with IPF who underwent spirometry, a non-significant association of the levels of mRNA and concentration p62 and Beclin1 with the results of the test of dynamically exhaled vital capacity, the percentage of total lung tumor, which can be discharged in 1 second, or the effusive capacity of the lungs of healthy controls, were observed. However, a tendency of decreased expression of p62 was observed for patients with IPF with FVC below 50.

Language

Greek

Issue date

2022-03-30

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

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487

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