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Identifier

000438180

Title

Ελεγχόμενη αποδέσμευση διφωσφονικών αντι-οστεοπορωτικών φαρμάκων από εδώδιμες πρωτεϊνικές γέλες ζελατινίνης

Alternative Title

Controlled release of bisphosphonate anti-osteoporotic drugs from edible protein gelatin gels

Author

Πετράκη, Νίκη Ε.

Thesis advisor

Δημάδης, Κωνσταντίνος

Reviewer

Μήλιος, Κωνσταντίνος
Σπύρος, Απόστολος

Abstract

The present work describes the synthesis of new edible, organic bovine gelatin hydrogels, which were used for the controlled release of a 1st generation bisphosphonate etidronic acid (ETID) (containing no N, but a methyl side chain) and its two derivatives, C₃BP (containing a propyl side chain) and C₅BP (containing a pentyl side chain). The studies were performed at different pH and temperature conditions, using additives or not. More specifically, two pH values were selected (4.00 and 7.00), and two temperatures T = 5 ^oC and T = 25 ^oC. The additives used were the weak polyelectrolyte polyacrylic acid (PAA, MB = 2000 Daltons) and SiO₂ nanoparticles. In order to evaluate the controlled release of the selected bisphosphonates, quantification was performed based on the peak area of the spectrum using proton and phosphorus Nuclear Magnetic Resonance Spectroscopy (¹1H and ³¹P NMR, 300 MHz). The results are plotted in diagrams containing the release curves of the respective bisphosphonate that correlate the % release versus time. It has been shown that the release of compounds from the edible gelatin hydrogels (which relatively low-cost) can be achieved. At the same time, it was generally observed that at a temperature of T = 25 ^oC, the release of bisphosphonates was higher relative to the temperature T = 5 ^oC. In addition, the gelatin matrix load did not appear to affect the release of bisphosphonates and no observable correlation was noted between molecular size and release. Finally, the presence of different alkaline earth ions Na⁺, K⁺, Li⁺, Cs⁺ did not cause any differentiation in the release of ETID.

Language

Greek

Subject

Bovine gelatin

Organic hydrogels

Osteoporosis

SiO2 nanoparticles

Διφωσφονικές ενώσεις

Ζελατίνη

Νανοσωματίδια διοξειδίου του πυριτίου

Οργανικές υδρογέλες

Οστεοπόρωση