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| Identifier |
000387781 |
| Title |
Μελέτη ενζύμων της πορείας των λιποξυγενάσων σε χλωροπλάστες |
| Alternative Title |
Study of the enzymes of lipoxygenase pathway in chloroplasts |
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Author
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Βαλσαμή, Ελευθερία-Αγγελική
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Thesis advisor
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Γανωτάκης, Δημήτριος
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Reviewer
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Τσιώτης, Γεώργιος
Κοτζαμπάσης, Κυριάκος
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| Abstract |
Fatty acid hydroperoxide lyase (HPL) is an important factor of the lipoxygenase path that is the defense system of plants against exogenous factors. HPL’s function leads to small molecular weight compounds production. They are products of great importance, such as 2-(E)-hexenal and hexanal. These substances belong to the "green leaf volatiles" and they are important precursors for plants. Despite all the studies done on the lipoxygenase pathway, the location of HPL in chloroplasts is still not known.
In the current study an effort of identification of the exact site of chloroplasts where the enzyme is placed was carried out. Isolation of the enzyme from spinach leaves was tried as well. For this reason different chloroplast membranes were isolated and tests of enzyme presence were conducted. In all samples that were isolated, kinetic tests were carried out in the presence of the enzyme’s substrate, 13- hydroyperoxide of linoleic acid, products was tested by the presence of 2,4-dinitro-phenylhydrazine, analysis with thin layer chromatography (TLC), spectroscopic determination, and SDS-PAGE electrophoresis. The main target was the determination of the presence of HPL or not. The spectrometric kinetic determination of the enzyme activity gave us good results. In the end, inhibition studies of the enzyme were carried out by the use of Methylene blue which resulted in 50% HPL’s action reduction.
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| Language |
Greek |
| Subject |
Fatty acid hydroperoxide lyase |
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Lipoxygenase pathway |
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Λυάση του υδρουπεροξειδίου των λιπαρών οξέων |
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Μονοπάτι λυποξυγάνασης |
| Issue date |
2014-11-21 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
95 |
Μελέτη ενζύμων της πορείας των λιποξυγενάσων σε χλωροπλάστες
