| Abstract |
Aim: The prevalence of non-organ specific autoantibodies and anti-parietal cells autoantibodies in patients with Chronic Hepatitis C. Material: 1) 142 sera from patients with Chronic Hepatitis C (HCV), 59 men ? 83 women with an average age of 61 years (range 27-96 years): 75 from Herakleion University Hospital, 17 from Rethymnon General Hospital, 25 from Perama Health Center, 25 from Spili Health Center, 2) 60 healthy blood donors from Rethymnon General Hospital, serum negative to hepatitis C virus (HCV): 46 men-14 women with an average age of 35 years (range 20-55 years). Method: Indirect Immunfluorescence (I-IFL) technique on human epithelial cells from larynx cancer (Hep-2 cells), Crithidia Luciliae and rat liver/kidney/stomach substrate (CT3). The initial dilutions were: for anti-nuclear autoantibodies-ANA, Golgi apparatus, spindle apparatus 1:80, against intermediate filaments-IMF, microfilaments-MF (Hep-2): 1:40, anti-dsDNA (Crithidia Luciliae) 1:10, antimitochondrial-AMA, antiribosomal-Rib, against liver-kidney microsomal-LKM, anti- liver cytosol-LC (CT3) 1:10, anti- smooth muscle autoantibodies-ASMA, anti- parietal cell autoantibodies-APCA, anti- reticulin autoantibodies-ARA (CT3) 1:40. Positive samples were diluted up to the highest positive title. Results: Serum autoantibodies were detected in 109 of 202 (53.96%) patients and control group members (healthy blood donors), in 104 of 142 (73.24%) patients and in 5 of 60 (8.33%) healthy blood donors. The most frequent autoantibodies in patients and healthy blood donors were ANA, positive in 76 (37.62%), ASMA, positive in 34 (16.83%), APCA, positive in 20 (9.9%), IMF, positive in 13 (6.44%). The most frequent autoantibodies in patients were ANA, positive in 72 (50.7%), ASMA, positive in 33 (23.24%), APCA, positive in 20 (14.08%), IMF, positive in 13 (9.15%). The most frequent autoantibodies in the control group (healthy blood donors) were ANA, positive in 4 (6.6%), ASMA, positive in 1 (1.67%). The prevailing dilution in HCV patients was 1:80 for ANA, 1:80 for ASMA and 1:40 for APCA, while the prevailing dilution in healthy blood donors was 1:80 for ANA and 1:40 for ASMA. Conclusions: 1) HCV possibly triggers immunological response mechanisms, provoking autoantibodies? appearance (73,24%). ANA (50,7%) and ASMA (23.24%) prevail according to our descriptive statistical analysis. 2) There is a limited correlation between APCA+ANA and sex in favor of women, ASMA+IMF and biopsy, ASMA+ANA and biopsy, IMF and biopsy. In addition there is a possible correlation between ANA and sex in favor of women, ANA and age in favor of ages 45-96 years, ANA+ASMA and age in favor of ages 45-96 years, ASMA and biopsy, ASMA+ANA and biopsy. Correlation was also found between IMF and biopsy. Moreover, there is a high correlation between ASMA and origin in favor of Health Centers, IMF and origin in favor of Health Centers, ASMA+IMF and origin in favor of Health Centers, ASMA+IMF and biopsy, IMF and biopsy. These correlations were established with statistical analysis of our data using Pearson?s X2, Group Statistical T-test and Oneway criterion. 3) The presence of autoantibodies with co estimation of their type and title and the patient?s clinical status leads towards the investigation of hepatic and extra-hepatic manifestations of possible autoimmune origin.
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Η έρευνα αυτοαντισωμάτων (ΑΑ) σε ασθενείς με χρόνια ηπατίτιδα C (HCV)
