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Identifier 000399740
Title Transvection μέσω μονωτών στη Drosophila melanogaster
Alternative Title Insulator-mediated transvection in Drosophila melanogaster
Author Βιτσάκη, Ηλέκτρα Γ
Thesis advisor Δελιδάκης, Χρήστος
Abstract Enhancers are regulatory elements that work primarily in cis. However, cases have been found where they take part in trans interactions, and one of them is transvection. Transvection is a phenomenon which, by taking advantage of the homologous pairing in somatic cells of Diptera, leads to the transcriptional regulation of a gene by enhancers and other regulatory sequences located in the homologous chromosome (in trans). Studies have shown that the GYI insulator can mediate transvection by pairing with its copy in the homologous chromosome. In this study we wanted to further study the correlation between insulators and transvection. First, we tested the efficiency of GYI-mediated transvection across the genome using a combination of two plasmid constructs with GYI which had been proven to cause transvection in the attp2 and attp40 site by previous studies. With the introduction of this combination in four more attP sites, we concluded that the GYI insulator can indeed cause robust transvection throughout the genome. Then we studied the contribution of GYI in ectopic expression of the reporter genes from neighbouring enhancers. We found that GYI can, at the same time, cause transvection and affect ectopic expression, either negatively or positively. Finally, we studied the putative involvement of other insulators, besides GYI, in transvection. For this purpose we made a two-reporter construct flanking one insulator and paired it with a two-enhancer construct flanking the same insulator. Specifically we worked with the insulators Fab-8 and scs'. These experiments showed that the insulator Fab-8 can cause strong transvection while scs' weak. Interestingly, we found that each of the two enhancers can only activate one of the reporters, indicating that transvection has directionality.
Language Greek
Subject Central nervous system
Disc
Enhancer
Eye
Fab-8
GYI
Gypsy
Scs
Wing
Δίσκος
Ενισχυτής
Κεντρικό νευρικό σύστημα
Μάτι
Μονωτής
Φτερό
Φόρα
Issue date 2016-03-18
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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