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Home    Διερεύνηση της γενετικής ποικιλότητας του δίθυρου Pinna nobilis στην Ανατολική Μεσόγειο και εκτίμηση του βαθμού επιμόλυνσής του από το παράσιτο Haplosporidium pinnae  

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Identifier 000427202
Title Διερεύνηση της γενετικής ποικιλότητας του δίθυρου Pinna nobilis στην Ανατολική Μεσόγειο και εκτίμηση του βαθμού επιμόλυνσής του από το παράσιτο Haplosporidium pinnae
Alternative Title Investigation of the genetic diversity of the bivalve Pinna nobilis in the Eastern Mediterranean and estimation of the extent of infection from the parasite Haplosporidium pinnae
Author Σαραφίδου, Γεωργία Κ.
Thesis advisor Παυλούδη, Χριστίνα
Reviewer Λαδουκάκης, Μανόλης
Κωτούλας, Γιώργος
Abstract The fan mussel Pinna nobilis Linnaeus, 1758 is an endemic species of the Mediterranean Sea included in the list of endangered species (Annex IV of the Habitat Directive and Annex II of the Barcelona Convention). It is one of the largest bivalves in the world, and plays an important role in the marine environment as an ecosystem engineer, yet it is threatened by various anthropogenic activities. Recently, mass mortality events (MME) have been detected among the P. nobilis populations, which are attributed mostly to a newly identified specific parasite, Haplosporidium pinnae. The assessment of genetic variation of P. nobilis and the evaluation of connectivity among the different populations, as well as the estimation of the parasite’s contamination are important elements for the protection and the conservation of this species. For this purpose, the COI and 16S rRNA genetic markers were chosen since they have been widely used in population genetics surveys and specifically for P. nobilis throughout the Mediterranean. Samples were collected using minimum invasion and nonlethal methods, to avoid stress induction in the individuals. Molecular techniques were used (DNA extraction, PCR amplification and Sanger sequencing) in order to reveal the patterns of genetic differentiation at the population level. For the sequences’ analysis various softwares were used, such as BioEdit, DnaSP and Network, including the sequences submitted to the GenBank platform. The results of this work show that there are common haplotypes in a Mediterranean scale and also different haplotypes within the same area. In the Eastern Mediterranean, low values of Fst were found, meaning little genetic divergence between the sampling areas: Lesvos, Attiki, Karpathos and Crete. This information depicts the connectivity between even remote locations and underlines the importance of the sea currents on migration and thus on the genetic flow.
Language Greek
Issue date 2019-11-29
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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