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| Identifier |
000376706 |
| Title |
Πρωτεομική ανάλυση της λευχαιμικής σειράς Κ562 παρουσία αναλόγων του gleevec |
| Alternative Title |
Proteomic analysis of K562 cell line in the presence of gleevec and its derivatives |
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Author
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Αρβανίτη, Κατερίνα
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Thesis advisor
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Τσιώτης, Γεώργιος
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| Abstract |
Chronic myelogenous leukemia (CML) is a malignancy of a pluripotent hematopoietic stem cell. This disorder arises from a reciprocal translocation between the long arms of chromosomes 9 and 22 known as the Philadelphia (Ph) chromosome. The molecular consequence of this translocation is the generation of a fusion protein BCR-ABL, a constitutively activated tyrosine kinase. Since tyrosine kinase activity is essential to the transforming function of BCR-ABL, an inhibitor of the kinase could be an effective treatment for CML.
Imatinib (Gleevec, Glivec, STI571 or CGP 57148) targets the tyrosine kinase domain of the oncogenic fusion gene BCR-ABL that causes CML and has revolutionized the treatment of the disease. However, a significant number of patients eventually develop resistance to it because their cancer cells are able to mutate and adapt. To overcome this resistance, new substances have been synthesized which are based on the structure of Gleevec.
In order to study the effect of the newly synthesized compounds we tested them in K-562 suspension cell line. We applied mass spectrometry together with stable isotope labelling by amino acids in cell culture (SILAC) for the comparative study of protein expression in these cells that were treated with a different derivative of gleevec every time. Heavy and light lysine and arginine were used for the SILAC labelling. Αnother approach was the use of an anti-phoshotyrosine antibody in order to check if it could bind phosphorylated proteins.
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| Language |
Greek |
| Subject |
Labeling mass spectometry |
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Proteomics Leykemia |
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SILAC |
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Λευχαιμία |
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Φασματομετρία μάζας |
| Issue date |
2012 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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| Views |
311 |
Πρωτεομική ανάλυση της λευχαιμικής σειράς Κ562 παρουσία αναλόγων του gleevec
