|
|
| Identifier |
000397266 |
| Title |
Gene expression study of hematopoietic stem cells in an experimental model of Systemic Lupus Erythematosus (SLE) |
| Alternative Title |
Μελέτη της γονιδιακής έκφρασης των αρχέγονων αιμοποιητικών κυττάρων σε πειραματικό μοντέλο Συστημάτων Ερυθηματώδους Λύκου |
|
Author
|
Γρηγορίου, Μαρία Γ.
|
|
Thesis advisor
|
Μπάνος, Α.
|
|
Reviewer
|
Μπέρτσιας, Γ.
Μπούμπας, Δ.
|
| Abstract |
Hematopoietic
Stem
Cells
(HSCs)
represent
a
cell
population
that
gives
rise
to
every
lineage
of
blood
cells,
including
granulocytes,
lymphocytes,
monocytes
and
endothelial
cells,
all
of
which
have
been
implicated
in
the
pathogenesis
of
Systemic
Lupus
Erythematosus
(SLE).
Our
working
hypothesis
is
that
the
fundamental
immune
aberrations
in
SLE
–genetic
or
epigenetic-‐
may
be
easier
to
be
traced
back
to
the
HSC
population.
Although
HSCs
are
thought
to
exist
in
a
dormant
state
within
the
bone
marrow
niche,
recent
evidence
suggests
that
in
lupus
mice
they
may
overproliferate
due
to
both
intrinsic
and
extrinsic
effects.
However,
the
molecular
identity
and
the
contribution
of
these
alterations
to
lupus
remain
elusive.
We
found
significantly
increased
frequencies
as
well
as
absolute
numbers
of
HSCs
in
the
BM
of
lupus
NZBxNZW/F1
mice
with
established
disease
as
compared
to
young
NZBxNZW/F1
mice
or
to
C57/BL6
mice.
Bone
marrow
populations
such
as
hematopoietic
stem
progenitors
cells
(HSPCs),
lymphoid
and
myeloid
lineages
seem
to
differ
in
homogeneity
depending
upon
either
age
or
disease,
indicating
an
alteration
in
HSC
potential
under
inflammatory
conditions.
Accordingly,
serum
from
F1
young
mice
promotes
healthy
HSCs
to
proliferation
and
skews
their
differentiation
to
myeloid
lineage.
Global
gene
expression
analysis
of
HSCs
originating
from
lupus
mice
revealed
547
differentially
expressed
(DEGs).
These
significantly
DEGs
contain
crucial
transcription
factors
for
hematopoiesis,
HSC
function
and
homeostasis,
as
well
as
regulators
for
immune
response
during
inflammation
and
autoimmune
diseases.
These
data
provide
initial
insights
in
the
fundamental
changes
of
HSC
in
lupus
within
the
inflammatory
milieu
of
the
disease.
|
| Language |
English |
| Subject |
HSCs |
|
RNA-Seq Autoimmunity |
|
SLE |
|
Αιμοποιητικά αρχέγονα κύτταρα |
|
Αυτοανοσία |
|
Συστηματικός Ερυθηματώδης Λύκος |
| Issue date |
2015-11-20 |
| Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
|
|
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Type of Work--Post-graduate theses
|
| Views |
263 |
Gene expression study of hematopoietic stem cells in an experimental model of Systemic Lupus Erythematosus (SLE)
