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Identifier

000369999

Title

Λειτουργική μελέτη μεταλλοπρωτεάσων εξωκυτταρίας στοιβάδας στο φορέα ελονοσίας Anopheles gambriae. Διερεύνηση του ρόλου τους στις αλληλεπιδράσεις του κουνουπιού με το παράσιτο της ελονοσίας

Alternative Title

Functional studies of Anopheles gambiae extracellular matrix metalloproteases. Implications in Plasmodium berghei sexual life cycle

Author

Γουλιελμάκη, Ευαγγελία

Thesis advisor

Λουκέρης, Αθανάσιος

Abstract

Midgut epithelial responses triggered by Plasmodium ookinete invasion in the vector Anopheles gambiae is a matter of intense research, due to their direct or indirect involvement in parasite survival. For this reason we undertook the effort to study mosquito metalloproteases of the extracellular matrix (ECM), which are the main enzymes involved in epithelial reorganization in several organisms. Taking advantage of gene predictions of Anopheles database, as well as, of phylogenetic relationships, we identified representative members of all three major families of ECM metallo-proteinases, ADAMs, ADAMTSs and MMPs. Transcriptional profiling indicated putative implications of ADAMs and ADAMTSs in developmental processes, however, an additional involvement of those proteases in vector-parasite interactions cannot be excluded. Our study was mainly focused on MMP-1, a representative member of MMP family of metalloproteases. Reverse genetics, cell biology and transcriptional profiling, as well as, the use of established Anopheles gambiae hemocyte-like cell lines, revealed not only involvement of AgMMP1 in the processes of midgut epithelial reconstitution after parasite invasion, as expected, but in addition a protective role of the AgMMP1 protease in the survival of the parasite during the oocyst stage. Our data indicate that these two roles are probably conferred by different MMP1 isoforms; a transmembrane and a secreted form, expressed in the mosquito midgut epithelium and hemocytes respectively.

Language

Greek

Subject

Midgut epithelium

Μεσεντερικό επιθήλιο

Issue date

2014-07-22

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses

Type of Work--Doctoral theses

Views

257

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