Post-graduate theses
Current Record: 14 of 802
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Identifier |
000460373 |
Title |
Development and reversal of Acute Myelomonocytic Leukemia (AMML) phenotype with an apparent effect on the male reproductive system |
Alternative Title |
Ανάπτυξη και αναστροφή φαινότυπου οξείας μυελομονοκυτταρικής λευχαιμίας με εμφανή επίδραση στο αρσενικό αναπαραγωγικό σύστημα |
Author
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Πατεράκη, Βασιλεία Ξ.
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Thesis advisor
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Αθανασάκη, Ειρήνη
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Reviewer
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Χαλεπάκης, Γιώργος
Ranella, Anthi
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Abstract |
Acute Myeloid Leukemia (AML) is a bone marrow disorder marked by genetic changes in blood cell
precursors, leading to abnormalities in hematopoietic stem cells. One rare variant of AML, known as
Acute Myelomonocytic Leukemia (AMML), is characterized by the presence of myeloid and
monocytoid cells in the peripheral blood and bone marrow. Notably, testicular infiltration in AML is
primarily observed in cases with myelomonocytic or monoblastic differentiation. Vaccination is a
method of immunotherapy that can actively stimulate a patient’s immune system to recognize and
destroy AML through introduction of a tumor antigen. Using a Leukemia-induced experimental
mouse model, the present study focused on the development and progression of the disease and
attempted to reverse the malignancy phenotype using an immunotherapy vaccination treatment
protocol. Further investigation into the impact of AML development on the male reproductive
system, specifically on testes, was also conducted, where WEHI-3B cell administration appeared to
cause an abnormal phenotype. The results presented here confirmed the immunogenicity of WEHI3B cells. Alterations in T cell phenotype and function were observed, reducing T and B cell levels, and
leading to immunological depletion. High expression of myeloid markers and effects on
immunosuppressive cytokine profiles were also detected, while leukemia bearing male mice
developed reproductive abnormalities at the testicular level. Τumor-Αssociated Antigen (TAAs)
immunotherapy vaccination appeared to affect the leukemic immunophenotype by restoring T and B
cell levels and lowering the immunosuppressive profile at the peripheral blood. The leukemiainduced phenotype in testes was also macroscopically reversed. Overall, immunotherapy vaccination
managed to stimulate specific immune responses and could be incorporated into a broader
immunotherapeutic approach, with positive aspects in the male reproductive system.
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Language |
English |
Subject |
Immunologyaml |
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Immunotherapy vaccine |
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TAAS |
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Ανδρικό αναπαραγωγικό |
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Ανοσοθεραπεία |
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Ανοσολογία |
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Εμβόλιο |
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Οξεία μυελογενής λευχαιμία |
Issue date |
2023-11-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/2/9/b/metadata-dlib-1699956295-455117-16126.tkl
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Views |
1193 |
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