Post-graduate theses
Current Record: 22 of 802
|
Identifier |
000460267 |
Title |
Investigation of the protective role of caloric restriction mimetics in myelin disruption : in vivo and in vitro approaches |
Alternative Title |
Διερεύνηση του προστατευτικού ρόλου μιμητών θερμιδικού περιορισμού στη διατάραξη της μυελίνης: in vivo and in vitro προσεγγίσεις |
Author
|
Καφφέ, Δέσποινα Β.
|
Thesis advisor
|
Καραγωγέως, Δόμνα
|
Reviewer
|
Βιδάκη, Μαρίνα
Ταβερναράκης, Νεκτάριος
|
Abstract |
Caloric restriction is defined as the reduction of food consumption without
malnutrition and is considered as a physiological inducer of autophagy that attenuates
demyelination and promotes remyelination. In this study we evaluated the effect of
nicotinamide (NAM) and resveratrol (RSV), two well-known caloric restriction mimetics
(CRMs), on myelin production upon demyelinating conditions as well as their direct effects on
the glial populations of the central nervous system that interact during the process of
remyelination. To address these questions we used a lysolecithin (LPC)-induced focal
demyelination mouse model as well as primary cultures of microglia, oligodendrocytes
and astrocytes. Preliminary data of our laboratory reveal that NAM results in increased
MBP immunoreactivity along with reduced microgliosis and astrogliosis at the lesion site
upon LPC-induced demyelination. Even though NAM does not directly affect the
proliferation or differentiation of oligodendrocyte progenitor cells (OPCs), it influences
both microglia and astrocytes. Specifically, NAM reduces the population of proinflammatory microglia and promotes the anti-inflammatory phenotype of astrocytes in
vitro, an effect that is, at least in part, mediated by autophagy. Similarly, RSV enhances
PLP density in the lesion site of LPC-demyelinated animals. However, it does not reduce
the accumulation of astrocytes or microglia at the area of the lesion. In fact, there is a
tendency of RSV to increase astrogliosis without affecting microglia accumulation.
Regarding its cell-autonomous effects, RSV does not affect either the proliferation or the
differentiation of OPCs, whereas it exerts ambiguous effects on the activation of
microglia and astrocytes, affecting the intermediate phenotypes. Overall, our results
indicate that both CRMs influence microglia and astrocytes directly, in favor of the
remyelination process. Our data indicate that NAM promotes a less inflammatory
microenvironment, whereas the effects of RSV on microglia and astrocytes are
multifaceted and require further investigation.
|
Language |
English |
Subject |
Astrocytes |
|
Autophagy |
|
Caloric restriction mimetics |
|
Demyelination |
|
Microglia |
|
Nicotinamide |
|
Oligodendrocytes |
|
Remyelination |
|
Resveratrol |
|
Απομυελίνωση |
|
Αστροκύτταρα |
|
Αυτοφαγία |
|
Επαναμυελίνωση |
|
Μικρογλοία |
|
Μιμητές θερμιδικού περιορισμού |
|
Νικοτιναμίδιο |
|
Ολιγοδενδροκύτταρα |
|
Ρεσβερατρόλη |
Issue date |
2023-11-24 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
|
|
Type of Work--Post-graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/8/1/9/metadata-dlib-1699358498-193386-26750.tkl
|
Views |
1048 |
Digital Documents
|
|
No permission to view document.
It won't be available until: 2026-11-24
|