Your browser does not support JavaScript!

Post-graduate theses

Current Record: 3 of 791

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000460373
Title Development and reversal of Acute Myelomonocytic Leukemia (AMML) phenotype with an apparent effect on the male reproductive system
Alternative Title Ανάπτυξη και αναστροφή φαινότυπου οξείας μυελομονοκυτταρικής λευχαιμίας με εμφανή επίδραση στο αρσενικό αναπαραγωγικό σύστημα
Author Πατεράκη, Βασιλεία Ξ.
Thesis advisor Αθανασάκη, Ειρήνη
Reviewer Χαλεπάκης, Γιώργος
Ranella, Anthi
Abstract Acute Myeloid Leukemia (AML) is a bone marrow disorder marked by genetic changes in blood cell precursors, leading to abnormalities in hematopoietic stem cells. One rare variant of AML, known as Acute Myelomonocytic Leukemia (AMML), is characterized by the presence of myeloid and monocytoid cells in the peripheral blood and bone marrow. Notably, testicular infiltration in AML is primarily observed in cases with myelomonocytic or monoblastic differentiation. Vaccination is a method of immunotherapy that can actively stimulate a patient’s immune system to recognize and destroy AML through introduction of a tumor antigen. Using a Leukemia-induced experimental mouse model, the present study focused on the development and progression of the disease and attempted to reverse the malignancy phenotype using an immunotherapy vaccination treatment protocol. Further investigation into the impact of AML development on the male reproductive system, specifically on testes, was also conducted, where WEHI-3B cell administration appeared to cause an abnormal phenotype. The results presented here confirmed the immunogenicity of WEHI3B cells. Alterations in T cell phenotype and function were observed, reducing T and B cell levels, and leading to immunological depletion. High expression of myeloid markers and effects on immunosuppressive cytokine profiles were also detected, while leukemia bearing male mice developed reproductive abnormalities at the testicular level. Τumor-Αssociated Antigen (TAAs) immunotherapy vaccination appeared to affect the leukemic immunophenotype by restoring T and B cell levels and lowering the immunosuppressive profile at the peripheral blood. The leukemiainduced phenotype in testes was also macroscopically reversed. Overall, immunotherapy vaccination managed to stimulate specific immune responses and could be incorporated into a broader immunotherapeutic approach, with positive aspects in the male reproductive system.
Language English
Subject Immunologyaml
Immunotherapy vaccine
TAAS
Ανδρικό αναπαραγωγικό
Ανοσοθεραπεία
Ανοσολογία
Εμβόλιο
Οξεία μυελογενής λευχαιμία
Issue date 2023-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/2/9/b/metadata-dlib-1699956295-455117-16126.tkl Bookmark and Share
Views 1163

Digital Documents
No preview available

No permission to view document.
It won't be available until: 2025-11-24