Post-graduate theses
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| Identifier |
uch.biology.msc//2004troullinaki |
| Title |
Μελέτη της επίδρασης των ενδοκυτταρικών μηχανισμών μεταφοράς και του ρόλου των ανταλλακτών Na+/Ca+2 (NCX1,2,3,4&5) κατά την νέκρωση στο C. elegans |
| Creator |
Troullinaki, Kostoula
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| Abstract |
Cell death can be categorized into genetically programmed and not genetically programmed. In the first case it takes the form of apoptosis. The apoptotic program is hardwired into the genetic material of every cell, but is activated only into those cells destinated to die at a given point in the time. This type of cell death is essential for the correct development of organs and tissues. Not genetically programmed cell death on the other hand, is the unanticipated destruction of a cell that under normal circumstances, was not destined to die. When cells stressed beyond their tolerance, they undergo this type of cell death, which is called necrosis. Numerous studies implicate necrotic cell death in many devastating human pathologies, such as stroke and neurodegenerative diseases. In the contrary to the apoptosis which has been extensively studied, little is known about the molecular mechanisms that bring about necrotic cell death. It seems that necrotic mechanisms did not evolve specifically to carry out necrosis. Recent studies demonstrate that under extremme circumstances, normal cellular activities are destabilized with devastating consequences for the cell. The earliest detectable abnormality of a dying cell is the formation of small, tightly wrapped membrane whorls that seem to originate at the plasma membrane. In the next steps, these worls are internalized and seem to coalesce into large, electro-dense membranous structures. The prominent internalized membranous inclusions indicate that intracellular trafficking might contribute to degeneration. Furthermore, disrupted trafficking has been implicated in neurodegenerative diseases, as Alzheimers disease, Huntingtons disease and ALS. Based on the above, we decided to study the presumable role of intracellular trafficking in the execution of necrotic cell death. For this reason, we used the nematode worm C. elegans. This model organism has been widely used in the discovery of molecular mechanisms of apoptosis and is the most popular for the study of neurodegeneration. Toxic mutations in several genes induce degeneration of specific types of neurons or other cells in the nematode. Cells that express these mutant genes undergo late-onset necrotic cell death, which is mechanistically and morfologically reminiscent of excitotoxic cell death in mammals. Based on the current bibliography, we decided to work on some genes encoding proteins that play key role in the pathways of intracellular trafficking in the mammals. We found their orthologs in C. elegans and we used their mutants in our studies on necrosis. In this way, we evaluated their contribution in the execution of necrotic cell death in the nmatode.
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| Issue date |
2004-11-01 |
| Date available |
2004-12-06 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/6/7/metadata-dlib-2004troullinaki.tkl
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| Views |
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