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Identifier 000460268
Title Functional cell-based assays for the characterization of putative insecticide resistance mutations in the β-adrenergic like octopamine receptor (Octβ2R) in arthropod pests
Alternative Title Λειτουργικές κυτταρικές δοκιμασίες για τον χαρακτηρισμό πιθανών μεταλλαγών ανθεκτικότητας σε εντομοκτόνα στον β-αδρενεργικό υποδοχέα οκτοπαμίνης (Octβ2R) σε αρθρόποδα παράσιτα
Author Κατσάνου, Αικατερίνη Α.
Thesis advisor Βόντας, Ιωάννης
Reviewer Δουρής, Βασίλειος
Γκουρίδης, Γεώργιος
Abstract Dermanyssus gallinae, also known as the Poultry Red Mite (PRM), is a destructive ectoparasite found in poultry farms, with a significant presence in Europe and other regions. These mites feed primarily on broiler birds, leading to economic losses in the egg production industry. PRM infestation has direct and indirect effects on poultry and human health. Direct effects include stress, decreased egg production, and quality, while indirect effects result from the transmission of viruses and bacteria. This poses a significant challenge for poultry welfare and food safety. Various methods have been investigated to control PRM populations. Among them are biological methods, physical interventions, vaccination approaches, chemical control and others have been studied. However, chemical approaches, while effective, face challenges such as resistance and food safety concerns. Amitraz, widely employed against various pests, has encountered resistance issues due to its extensive use. Resistance to amitraz is associated with genetic mutations in octopamine receptor genes, affecting the efficacy of the acaricide. This resistance has been observed in mite species like Tetranychus urticae and Dermanyssus gallinae. Understanding the genetic and molecular basis of resistance is crucial for developing effective control strategies. This thesis aims to validate the impact of β-adrenergic-like octopamine receptor A89V mutation identified in amitraz-resistant PRM populations (Katsavou E unpublished data). Our methodology involved the cloning of OAR genes, transfection optimization, establishment of stable cell lines, and activity assays to evaluate the impact of the A89V mutation on the activation of Octβ2R upon binding of amitraz and DPMF. Concluding, statistical analysis revealed that there are significant differences between the activity levels of the wild-type receptor and the one carrying the A89V mutation, in the presence of either amitraz or DPMF. Notably, we developed a heterologous protein system in HEK293 cells, that can be utilized for the investigation of various resistance mechanisms.
Language English
Subject Activity assays
Mammalian cells
Parasites
Issue date 2023-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/0/c/2/metadata-dlib-1699359338-904260-26923.tkl Bookmark and Share
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