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Identifier 000458556
Title Developmental differences in neuronal properties of cortico-cortical and cortico-amygdalar projecting neurons of the mouse female and male PFC
Alternative Title Αναπτυξιακές διαφορές των νευρωνικών ιδιοτήτων φλοιοφλοιϊκών και φλοιοαμυγδαλικών προβλητικών νευρώνων του προμετωπιαίου φλοιού θηλυκών και αρσενικών μυών
Author Ιορδανίδου, Χρυσούλα Α.
Thesis advisor Σιδηροπούλου, Κυριακή
Reviewer Φρουδάκης, Εμμανουήλ
Σμυρνάκης, Στέλιος
Abstract Until recently, neuroscience research focused on the anatomical, physiological, and functional properties of individual neurons. However, neurons do not work in isolation: they work together in neuronal circuits. Understanding brain-wide neuronal communication through specific patterns of long-range synaptic connectivity is a step closer to understanding cognition, behavior and emotion, and how these get impaired in neuropsychiatric disorders. The prefrontal cortex (PFC) is the so-called "executive center" of the brain involved in high-level cognitive functions, emotion, attention and perception. Impairments of the PFC are implicated in diverse neuropsychiatric disorders, including schizophrenia, attention deficit hyperactivity disorder (ADHD), addiction, and depression. Wholebrain connectivity studies have shown that the mouse PFC has extensive connections to nearly all brain areas; it receives a variety of long-range excitatory inputs and sends diverse outputs (Gao et al., 2022, Anastasiades & Carter, 2021). Additionally, adaptations in synaptic activity and connectivity during development are critical to proper circuit wiring, and synaptic impairments during development can lead to circuit dysfunction and disorders. Therefore, studying neuronal circuits in different developmental levels is essential for understanding the formation of circuits and their role in cognitive functions and emotional behavior. Here, we combined circuit-tracing approaches and whole-cell patch clamp recordings to study the intrinsic excitation and inhibition properties of a single neuronal population (L2/3 pyramidal neurons of the mouse PFC) projecting to two distinct brain areas: the contralateral PFC hemisphere (cPFC) or the amygdala (AMY). We choose these two projections as they are involved in PFC-mediated cognitive functions and emotional regulation, respectively. To dissect the cortico-cortical and cortico-amygdalar neurons, a retrograde fluorescent substance (cholera toxin B) was stereotaxically injected into the PFC or AMY of mice at different developmental stages (adolescent, adult). Whole-cell patch clamp recordings of the projecting PFC neurons revealed a significant effect of the developmental stage on the spontaneous excitatory postsynaptic current (sEPSC) frequency of cortico-amygdalar projections. An overall tendency of higher excitation/inhibition (E/I) ratio was observed in adult compared to adolescent mice, in both circuits. Overall, this study provides useful insights in the cortico-cortical (PFC→cPFC) and corticoamygdalar (PFC→BLA) projections of L2/3 pyramidal neurons of the prelimbic area of the prefrontal cortex, across development and in both sexes.
Language English
Subject Amygdala
Development
Mice
Prefrontal cortex
Projecting neurons
Αμυγδαλή
Ανάπτυξη
Μύες
Προβλητικοί νευρώνες
Προμετωπιαίος φλοιός
Issue date 2023-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/4/a/9/metadata-dlib-1694681710-256155-9514.tkl Bookmark and Share
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