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Identifier 000459675
Title Study of in vitro differentiation of NE-4C cells encapsulated in 3D adECM hydrogels
Alternative Title Μελέτη της in vitro διαφοροποίησης των ενθυλακωμένων NE-4C κυττάρων σε 3D υδρογέλες που προέρχονται από λιπώδη ιστό
Author Σταμπούλη, Κυριακή
Thesis advisor Ρανέλλα, Ανθή
Reviewer Χαλεπάκης, Γιώργος
Στρατάκης, Μανώλης
Abstract The interaction between extracellular matrix (ECM) and cells influences several cellular functions such as survival, proliferation, differentiation, and migration. Cell growth within 3D scaffolds resembling cellular microenvironments provides better insights into tissue development than traditional 2D culture systems. Hydrogels, a type of polymeric material, offer not only physical and chemical properties that support cell development, but also 3D microarchitecture that creates a more physiologically relevant environment, favoring tissue regeneration and mechanosensing, particularly in the neural regeneration studies. Among different studies, in vitro systems with cells encapsulated in 3D hydrogels appear to better replicate the neural tissue microenvironment, facilitating the investigation of the viability and differentiation process of neural stem cells (NSCs). In this study, we aimed to investigate the viability and differentiation process of NSCs by employing in vitro systems with neuroectodermal NE-4C cells encapsulated in 3D hydrogels derived from adipose tissue ECM (adECM). By varying the concentration of the adECM in the hydrogels (7.5 and 10 mg/mL), the impact on cell behavior and differentiation pathways was examined. Results showed that there are differences in structure of fibers and pore formation between two hydrogels. Even though these differences did not significantly affect cell survival, they did influence the differentiation process as revealed by the relative expression of neuronal lineage specific markers such as tubulin βΙΙΙ, synaptophysin, and gfap. Understanding the in vitro differentiation of NE-4C cells encapsulated in 3D adECM hydrogels has the potential to advance the development of in vitro models for neural tissue engineering and regenerative medicine.
Language English
Subject 3D in vitro model
3D μοντέλο in vitro
Cell fata
Extracellular matrix
Neural stem cells
Εξωκυττάρια μήτρα
Μοίρα κυττάρου
Νευρικά βλαστικά κύτταρα
Issue date 2023-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/5/6/0/metadata-dlib-1697727269-740353-17526.tkl Bookmark and Share
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