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Identifier

000460387

Title

Responsive protein-polymer conjugate nanoparticles for drug delivery

Alternative Title

Νανομεταφορείς αποκρίσιμων βιοσυζύγων πρωτεΐνης-πολυμερούς για μεταφορά φαρμάκων

Author

Χαριτάκη, Μυρτώ Ε.

Thesis advisor

Βελώνια, Κέλλυ

Reviewer

Φαρσάρη, Μαρία
Ρανέλλα, Ανθή

Abstract

Nanoparticles (NPs) as Drug Delivery systems have drawn significant interest in the field of nanomedicine. Responsive nanoparticles in particular are a very promising class of drug delivery nanovesicles with the ability to release a therapeutic cargo under specific stimuli. Targeted drug release from responsive nanoparticles can be a useful tool to the drug administration in the acidic environment of cytoplasm in cancer cells as well as low pH of different organelles (lysosomes). The research performed during this study was based on the development and evaluation of three different types of responsive NPs (pH-, temperature- and dual- responsive NPs) and in particularly focused on the drug loading and release study of pH-responsive NPs. More specifically different monomers (i.e., DMAEMA, DPA and NIPAM) were grafted from albumins (has and BSA) via an oxygen tolerant, copper-mediated controlled radical polymerization approach, resulting in responsive self-assembled nanoparticles. The response of albumin- responsive polymer NPs was studied under specific stimuli (pH or temperature). Model-drug dye molecules such as Eosin Y, fluorescein and acridine orange and a model therapeutic enzyme (pepsin) and chemotherapeutic drugs (5-fluorouracil, oxaliplatin, natural compounds with anticancer properties), were encapsulated in the responsive nanoparticles in the scope of studying their encapsulation efficiency, loading capacity and drug release.

Language

English

Subject

Bioconjugates

Responsive polymersP

Αποκρίσιμα πολυμερή

Βιοσυζυγή

Νανοσωματίδια

Issue date

2023-11-24

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses