Post-graduate theses
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Identifier |
000459675 |
Title |
Study of in vitro differentiation of NE-4C cells encapsulated in 3D adECM hydrogels |
Alternative Title |
Μελέτη της in vitro διαφοροποίησης των ενθυλακωμένων NE-4C κυττάρων σε 3D υδρογέλες που προέρχονται από λιπώδη ιστό |
Author
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Σταμπούλη, Κυριακή
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Thesis advisor
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Ρανέλλα, Ανθή
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Reviewer
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Χαλεπάκης, Γιώργος
Στρατάκης, Μανώλης
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Abstract |
The interaction between extracellular matrix (ECM) and cells influences several cellular
functions such as survival, proliferation, differentiation, and migration. Cell growth within 3D
scaffolds resembling cellular microenvironments provides better insights into tissue
development than traditional 2D culture systems. Hydrogels, a type of polymeric material, offer
not only physical and chemical properties that support cell development, but also 3D
microarchitecture that creates a more physiologically relevant environment, favoring tissue
regeneration and mechanosensing, particularly in the neural regeneration studies. Among
different studies, in vitro systems with cells encapsulated in 3D hydrogels appear to better
replicate the neural tissue microenvironment, facilitating the investigation of the viability and
differentiation process of neural stem cells (NSCs). In this study, we aimed to investigate the
viability and differentiation process of NSCs by employing in vitro systems with
neuroectodermal NE-4C cells encapsulated in 3D hydrogels derived from adipose tissue ECM
(adECM). By varying the concentration of the adECM in the hydrogels (7.5 and 10 mg/mL), the
impact on cell behavior and differentiation pathways was examined. Results showed that there
are differences in structure of fibers and pore formation between two hydrogels. Even though
these differences did not significantly affect cell survival, they did influence the differentiation
process as revealed by the relative expression of neuronal lineage specific markers such as
tubulin βΙΙΙ, synaptophysin, and gfap. Understanding the in vitro differentiation of NE-4C cells
encapsulated in 3D adECM hydrogels has the potential to advance the development of in vitro
models for neural tissue engineering and regenerative medicine.
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Language |
English |
Subject |
3D in vitro model |
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3D μοντέλο in vitro |
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Cell fata |
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Extracellular matrix |
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Neural stem cells |
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Εξωκυττάρια μήτρα |
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Μοίρα κυττάρου |
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Νευρικά βλαστικά κύτταρα |
Issue date |
2023-11-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/5/6/0/metadata-dlib-1697727269-740353-17526.tkl
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Views |
991 |
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