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Identifier

000453095

Title

Designing tools for discovering RNF20/40 mode of action in transcription regulation

Alternative Title

Σχεδιασμός εργαλείων για την ανακάλυψη του τρόπου δράσης του RNF20/40 στη ρύθμιση της μεταγραφής

Author

Θεοδοσίου, Βάιος Α.

Thesis advisor

Lavigne, Matthieu

Reviewer

Γιακουντής, Αντώνιος
Παυλόπουλος. Αναστάσιος

Abstract

Post-translational modifications (PTMs) of histones play a central role in regulating all nuclear processes requiring access to DNA in all eukaryotic cells and are extremely important in keeping human cells healthy. Recent studies suggest that the balanced activity of RNF20/40 (E3-Ubiquitin ligase) and the de-ubiquitination (DUB) module of SAGA determines the structural properties of histone H2B and controls transcription elongation. The evidence that RNF20/40 potentially binds nascent RNA directly raised our interest to determine if nRNA shape and size can influence H2Bub-associated functions. Furthermore, findings that loss of RNF20/40 and H2B monoubiquitylation (H2Bub) is observed in several cancers and is linked to an aggressive phenotype, and is also an indicator of poor prognosis. In this study, we aimed to create important molecular and cellular tools that will be important to map RNF20 DNA and RNA binding sites. Preliminary loss-of-functions or overexpression results also suggest that RNF20 functions are essential in Huh7 cells and determine the growth of many cancer cell lines. This thesis sets the stage for the characterization of how RNF20 is recruited and operates in the genome.

Language

English

Subject

Cancer

HUH7 cells

HUH7 κύτταρα

Η2ΒUB

Καρκίνος

Issue date

2023-03-24

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses