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Identifier

000452157

Title

The effect of Cellulose Acetate (CA) micropatterned replicas’ topography, loaded with statins, on osteogenic differentiation of mouse Mesenchymal Stem Cells (MSCs)

Alternative Title

Η επίδραση της τοπογραφίας των μικροδομημένων ρεπλικών οξικής κυτταρίνης, ενσωματωμένες με στατίνες, στην οστική διαφοροποίηση μεσεγχυματικών βλαστικών κυττάρων ποντικού

Author

Κανακουσάκη, Ελένη Ε.

Thesis advisor

Ρανέλλα, Ανθή

Reviewer

Αθανασάκη, Ειρήνη
Βελόνια, Κέλλυ

Abstract

Bone disorders are considered a major health, economic and social issue around the world. Although advancements have been made in contemporary medicine to surpass the difficulties in bone healing and regeneration, drawbacks still exist. Therefore, Bone Tissue Engineering remains an emerging field in medicine, attempting to combine biocompatible materials and stem cells for alternative implants in order to avoid the donor’s implant rejection. Latest advancements in this field, suggest that the surface topography of such implants, plays a mechanotrsanductive role by regulating the cells’ responses and functions. Among other techniques, soft lithography is reported as an easy and repeatable method to replicate micropatterned polymeric scaffolds. Furthermore, various polymers, natural or synthetic, have been used as biomaterials for tissue engineering applications, though with different capabilities. Additionally, in many reported cases, small molecules or drugs are incorporated into the produced scaffolds in order to enhance desirable outcomes. In the present master thesis, Cellulose Acetate (CA), a natural polymer, was used as biomaterial for the production of scaffolds, due to its biocompatibility, biodegradability, safety, low cost and excellent mechanical properties. Two types of micro-patterned surfaces and a non-patterned (flat) surface of the CA scaffolds were produced via soft lithography. During the production method, two statins (simvastatin and rosuvastatin) were also incorporated into the scaffolds, in order to further investigate their reported osteo-inductive role. Finally, the cell culture studies were carried out using mouse Mesenchymal Stem Cells (mMSCs), an important cell source in tissue engineering due to their ability to self-renew, proliferate, and differentiate into a wide range of tissuespecific lineages, including chondrogenic, adipogenic and osteogenic lineages. The purpose of this study is to investigate the effect of flat and micropatterned CA scaffolds with or without statins, on mMSCs morphology, adhesion, proliferation and osteogenic differentiation, aiming to produce scaffolds with low environmental footprint in order to guide cellular responses, for the field of Bone Tissue Engineering.

Language

English

Subject

Cell adhesion

Mechanotransduction

Soft lithography

Tissue engineering

Topography

Κυτταρική προσκόλληση

Μαλακή λιθογραφία

Μεσεγχυματικά βλαστικά κύτταρα ποντικού

Μηχανική ιστών