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Identifier uch.biology.msc//2004vrettos
Title Τεκμηρίωση και χαρακτηρισμός του ρυθμιστικού ρόλου του miR-13
Creator Vrettos, Nikolaos
Abstract MicroRNAs are small single stranded RNA molecules, of about 22 nucleotides, that can regulate important cellular functions in animals and plants. They exert their regulatory role by targeting mRNAs for cleavage or translational impairment. Hence they are regulators of gene expression at the post-transcriptional level. The subfamily of K-box miR-2/miR-13 in Drosophila contains members with almost identical sequence, and consequently similar functional roles. The gene CG10222 carries a K-box motif at its 3΄UTR with which it is potentially down regulated by members of the K-box subfamily. During this project heterologous sensor plasmids were constructed containing fusions between EGFP protein and the wild type 3΄UTR of CG10222 as well as mutated versions of it. These mutations aimed either to weaken or reinforce the interaction between miRNA and mRNA. All transcripts were under the control of a constitutively active tubulin promoter. The sensor constructs were used for the generation of stable Drosophila transgenic lines. EGFP fluorescence analysis at 3rd instar larvae and wing imaginal discs was followed. It was concluded that indeed miR-13b is a negative regulator of CG10222. The region between 11-24 nucleotides after the stop codon at the 3΄UTR was found to be a regulatory element that interacts with miR-13b. Additionally, it was observed that miR-13b is present throughout the body of the larva except for the male gonads. CG10222 has an unidentified function. RNAi strategies against it show that it is indispensable for embryo physiology. Finally there is evidence for a testis specific expression.
Issue date 2004-11-01
Date available 2005-01-10
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/0/a/f/metadata-dlib-2004vrettos.tkl Bookmark and Share
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