Your browser does not support JavaScript!

Post-graduate theses

Current Record: 627 of 800

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier uch.biology.msc//2003giakoumakis
Title Ο Πιθανός Ρόλος του Καταβολισμού των Πολυαμινών και των Ενεργών Μορφών Οξυγόνου κατά την Καταπόνηση Αλατότητας σε Κυτταροκαλλιέργεια Vitis vinifera cv Soultanina και κατασκευή πλασμιδιακών φορέων για την απενεργοποίηση του γονιδίου της αποκαρβοξυλάσης της S-Αδενοσύλ-Μεθειονίνης
Creator Giakoumakis, Dimitris
Abstract Salinity is a complex environmental factor incorporating osmotic, and ionic stress as well as secondary stress factors such as oxidative stress. It has been documented that salt stress can lead to the formation and accumulation of reactive oxygen species (ROS), and to programmed cell death (PCD). At this work, an effort has been made to study the polyamine catabolism in Vitis cell cultures (Vitis vinifera cv Soultanina) during salt stress. It was hypothesized that ROS generation, through PAO activation, can trigger PCD in salt-treated cell cultures. The results showed: i) reduction of cell viability, ii) small increase of PAO specific activity, iii) fragmentation of genomic DNA (indication of PCD), iv) no significant generation of ROS. Furthermore, plasmid vectors were constructed in an effort to silence the S-adenosyl-methionine decarboxylase gene, through a double incorporation of the same cDNA fragment in opposite orientation separated by an intron sequence between them (pan handle) for in vivo splicing. The final construct comprises a powerful tool for the study of polyamines-ethylene interaction during senescence, and of the role of polyamines in response to salt stress.
Issue date 2003-11-01
Date available 2003-11-27
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/9/0/8/metadata-dlib-2003giakoumakis.tkl Bookmark and Share
Views 235

Digital Documents
No preview available

Download document
View document
Views : 8