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Identifier

uch.biology.msc//2002missiou

Title

Δημιουργία γενετικά τροποποιημένων φυτών πατάτας που φέρουν ανθεκτικότητα στον ιό της πατάτας Y (Potato virus, PVY)

Author

Μίσσιου, Αναστασία

Thesis advisor

Τσαγρή, Ευθυμία

Thesis advisor

Ρουμπελάκη-Αγγελάκη, Καλλιόπη

Abstract

The generation of transgenic plants that are resistant to several viruses has been reported frequently. In a number of cases the resistance was a result of the introduction of virus-derived genes. The mechanism underlying this form of resistance appears to be a form of post-transcriptional gene silencing (PTGS). PTGS is characterized by the recognition of transgene target in the cytoplasm followed by its highly specific degradation. Double-stranded RNA (dsRNA) molecules possess a key role in this mechanism. These molecules target and lead to degradation of the corresponding homologous mRNA in the cell. The construction and introduction of such double stranded RNA molecules in the plant genome results in the specific silencing of transgene, endogens and viruses. Potato virus Y (PVY) is one of the most destructive viruses in potato production causing severe losses in potato yield. Based on the mechanism of silencing, in this work we try to generate potato transgenic lines that are resistance to PVY. These plants were transformed with a DNA construct named PH-PVY 9100, containing an inverted repeat of a fragment of a CP-gene of the viral genome, which led to the transcription of RNA able to form of a double stranded RNA. This form of RNA is target of specific Rnases and further induces the mechanism of PTGS. PTGS led to the production of siRNA, which in a most of cases are able to suppress the viral infection. Thirty-two transgenic lines were generated with the above construct. We tested 19 different transgenic lines for the presence of the transgene with Southern blot analysis and we found that in 14 of these lines the transgene was present. Furthermore in 18 out of 26 checked transgenic lines the siRNA were detected. In general, there was a correlation between resistance and the presence of siRNA in transgenic lines. The resistance in tested transgenic plants was estimated in 75%. These results are promising and remain to test if this form of resistance is stable and efficient in field condition.

Language

Greek

Issue date

2002-11-22

Date available

2002-10-11

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link

https://elocus.lib.uoc.gr//dlib/4/a/d/metadata-dlib-2002missiou.tkl