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Identifier |
000451006 |
Title |
Development of a combined surface plasmon resonance and Love wave - surface acoustic wave system for biophysical studies |
Alternative Title |
Ανάπτυξη συστήματος που συνδυάζει τις τεχνικές συντονισμού επιφανειακών πλασμονίων και επιφανειακών ακουστικών κυμάτων τύπου Love για βιοφυσικές μελέτες |
Author
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Σαμαρεντσής, Αναστάσιος Γ
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Thesis advisor
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Γκιζελή, Ηλέκτρα
|
Reviewer
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Francis, Laurent A.
Friedt, Jean Michel
Χαλεπάκης, Γιώργος
Αθανασάκη, Ειρήνη
Gogolides, Evangelos
Mitsakakis, Konstantinos
|
Abstract |
The field of biosensing is closely related to the development of sensitive analytical
techniques. Biosensors can be defined as such tools, incorporating a sensitive biological
element and a transducer for the detection of a target analyte. Despite the great advances in
the field of biosensors, there is still an ongoing demand for more accurate analytical
information. In this PhD research project, we present the development of a novel combined
surface plasmon resonance (SPR) and Love wave (LW) surface acoustic wave (SAW) system for
biophysical studies. To achieve the above, we first investigated the experimental parameters
required to match simultaneously the conditions for efficient SPR coupling and acoustic
waveguiding. A hybrid SPR/LW-SAW device was designed and fabricated incorporating a
PMMA waveguide layer and a Au-coated sensing area. The sensor was evaluated in terms of
materials and structures in order to identify the optimal thickness for the PMMA and Au
layer. In addition to that, a fluidic system consisting of a 3D-printed plastic holder integrated
with a PDMS microfluidic chip was developed to allow experiments under continuous flow.
The system was calibrated in a systematic study of the optical and acoustic responses for
different surface perturbations i.e., rigid mass loading (Au deposition) and pure viscous
loading (glycerol and sucrose solutions). Subsequently, simultaneous optical and acoustic
measurements were employed for monitoring biomolecular interactions on a single surface.
First, the combined SPR/LW-SAW platform was used to study the adsorption of proteins on
Au. The proteins tested include bovine serum albumin (BSA) and three biotin-binding proteins;
avidin (Av), streptavidin (SAv) and neutravidin (NAv). We proved that through appropriate
quantitative interpretation of the combined data, it is feasible to extract protein film
properties not accessible with a single technique. Finally, the system was applied for the
characterization of double-stranded DNA molecules (dsDNAs) of various sizes immobilized on
a SAv-modified, Au surface through a SAv-biotin link. Acoustic and optical signals were
analyzed to get further insight into the properties of the immobilized DNA molecules
including surface mass, conformation status and water content. Having verified the abovementioned characteristics, we believe that the developed platform could lead to improved
understanding of dynamic processes at biointerfaces.
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Language |
English |
Subject |
Biosensors |
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Biotin-binding proteins (Avidin, Streptavidin and Neutravidin) |
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Combined SPR/LW-SAW biosensing platform |
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Double-stranded DNA molecules (DSDNA) |
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Protein adsorption |
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Βιοαισθητήρες |
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Βιοφυσική μελέτη |
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Επιφανειακά ακουστικά κύματα τύπου Love (LW-SAW) |
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Προσρόφηση πρωτεϊνών |
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Πρωτεΐνες που προσδένουν Βιοτίνη (Αβιδίνη, Στρεπταβιδίνη και Νιουτραβιδίνη) |
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Συντονισμός επιφανειακών πλασμονίων (SPR) |
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Χαρακτηρισμός μορίων δίκλωνου DNA (DSDNA) |
Issue date |
2022-10-07 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
|
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/1/e/b/metadata-dlib-1662720880-104933-3708.tkl
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Views |
438 |