Doctoral theses

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Identifier	uch.biology.phd//1997DIS0026
Title	Προσεγγίσεις στην ενζυμική μετατροπή της Χιτίνης σε Χιτοζάνη-Ετερόλογη έκφραση της Απακετυλάσης της Χιτίνης του μύκητα Μucor Rouxil και γενετική μελέτη δυο απακετυλασών της χιτίνης απο τον μύκητα Saccharomyces Cerevisiae
Alternative Title	Bioconversion of Chitin to Chitosan:Heterologous expression of Mucor Rouxil chitin Deacetylase and genetic analysis of Ssccharoyces Cerevisiae chitin deacetylases
Author	Χριστοδουλίδου, Αννα Γ.
Thesis advisor	Μπουριώτης, Βασίλης
Abstract	Chitosan is a biopolymer used for various biotechnological applications. An attractive biotechnologucal procedure for the production of chitosan is the conversion of chitin to chitosan by the enzyme chitin deacetylase.The aim of this dissertation was the heterologous expression of the well characteri- sed chitin dealetylases from the fungus Mucor Rouxil and the molecular and biological characterization of two new chitin deacetylases from the yeast Saccharomyces Cerevisiae.Succesful expression of the enzyme was achieved in the methylotropic yeast Pitsia Pastoris.The properties of the recombinant enzyme were comparable to those of the native enzyme while additionally the enzyme was secreted in almost pure form and sufficient yield(~50gr/l). Two new chitin deacetylases were cloned from saccharomyles cerevisiae. Their expression is sporulation specific and they are required for the proper formation of the ascospore walls
Language	Greek
Subject	Χιτοζάνη; Απακετυλάση της χιτίνης; Ετερόλογη έκφραση; Σποριοειδική έκφραση; Τοίχωμα ασκοσποριών; Chitosan; Chitin deacetylase; Heterologous expression; Sporulation specific expression; Ascospore walls
Issue date	1997-02-06
Collection	School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
	Type of Work--Doctoral theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/f/2/7/metadata-dlib-1997DIS0026.tkl
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