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Identifier uch.biology.phd//1997DIS0022
Title Μελέτη της ενζυμικής δραστηκότητας απακετύλασης της χιτίνης
Alternative Title Study of chitin deacetylase enzyme activity
Author Τσίγκος, Ιάσoνας Ιωάννης
Thesis advisor Μπουριώτης, Βασίλης
Abstract The present doctoral dissertation focuses on the study of chitin deacetylase activity, continuing the effort towards the desigh of an enzymatic process for the deacetylation of chitin. A new chitin deacetylase was purified to homogeneity from the culture filtare of the fungus Colletotrichum lindemuthianum and futher characterized. The mode of action of chitin deacetylase from the fugus Mucor rouxii was studied on water soluble chitin oligomers. Two different alternative proccedures were used; Mass spectroscopy and the sequencial action of two exo-glycosidases in conjuction HPLC. Futhermore, the gene coding for the synthesis of a chitinase was cloned from the psychrophilic bacterium Arthrobacter spTAD20. Interestingly, it is the first chitinase gene ever cloned from a psychrophilic microorganism
Language Greek
Subject Χιτίνη; Aπακετύλαση της χιτίνης; Oλιγομερή χιτίνης; Mηχανισμός δράσης; Xιτοζάνη; Xιτίναση
Issue date 1997-02-06
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link https://elocus.lib.uoc.gr//dlib/c/6/3/metadata-dlib-1997DIS0022.tkl Bookmark and Share
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