Doctoral theses
Current Record: 311 of 340
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Identifier |
uch.biology.phd//1995papanikolaou |
Title |
Ο ρόλος των μειωτών ιοντικής ισχύος στη διαλυτότητα των βιομορίων στα μικτά υδατικά ηλεκτρολυτικά διαλύματα και εφαρμογές στη χρωματογραφία και στην κρυστάλλωση |
Alternative Title |
The role of ionic strength reducers in the solubility of biomolecules in mixed aqueous electrolytic solutions and applications to chromatography and crystallization |
Author
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Παπανικολάου, Ιωάννης Κ
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Thesis advisor
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Γανωτάκης, Δημήτρης
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Abstract |
We define as Ionic strength reducers or simply reducers the organic solvents, PEG and all other substances that reduce the ionic strength of an electrolyte solution. The reducers, by decreasing the dielectric constant of a solution, induce the formation of neutral ion pairs, which results in reduced ionic strength. Green's equation describes the solubility of biomolecules in an electrolyte solution as a function of the ionic strength. We have formulated the generalized Green's equation, the solubility of biomolecules in the generalized salting in increases, when the electrolyte concentration increases and or the reducer concentration decreases and vise versa. And the solubility of macromolecules in the generalizedsaltingout increases, when the electrolyte concentration decreases and or the reducer concentration increases and vise versa. As a result of the generalized Green's equation, elution from Ion exchange matrices is included by the increase of the electrolyte concentration and or by the decrease of the reducers concentration and vise versa. In conclusion, in aqueous electrolyte solutions, the addition of ionic strength reducers increases electrostatic interactions and decreases polar interactions.
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Language |
Greek |
Subject |
Βιομόρια; Διαλυτότητα; ΕΝΑΛΑΤΩΣΗ; ΕΞΑΛΑΤΩΣΗ; Κρυστάλλωση; Οργανικοί διαλύτες; Χρωματογραφία |
Issue date |
1995-12-07 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/1/0/2/metadata-dlib-1995papanikolaou.tkl
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Views |
356 |