Post-graduate theses
Current Record: 241 of 291
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| Identifier |
uch.physics.msc//2006drakakis |
| Title |
Κατασκευή Τρισδιάστατων Μικροδομών Βιολογικών Μορίων |
| Alternative Title |
Fabrication of 3D Biomolecular Microstructures |
| Creator |
Drakakis, Theodore
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| Abstract |
We demonstrate a novel method for three-dimensional patterning of proteins and other biological molecules. The technique is based on multi-photon polymerization of photosensitive composites and biotin-avidin chemistry. This technique opens the way for the fabrication of structures with a wide range of biomaterials, as well as studying their dynamics within complex 3D structures. For the fabrication of the 3D structures, multiphoton polymerisation is used. Multiphoton polymerization of photo-sensitive polymers is a very promising approach for the fabrication of complicated three-dimensional microstuctures. Compared with projection lithography (photo, x-ray and electron beam) and soft lithography technologies, multi-photon photopolymerization is unique in its 3D processing capability. When femtosecond laser pulses are tightly focused into the volume of a photosensitive resin, which is transparent in the infrared, the liquid is photopolymerized into solid state by absorbing more than one photons. This process is confined to a highly localized area at the focal point due to the nonlinear dependence of the polymerisation rate on the laser intensity. However to establish this technology as a micro processing tool , a lot of work on optics , materials and electronic controlling has been done to reduce the writing laser power ,and improve the fabrication accuracy and efficiency. Biotin is subsequently attached to the 3D structures via UV-activated cross-linking. The integrity of the photolytically immobilized biotin is confirmed by detecting the binding of fluorescently labeled avidin via fluorescence. For monitoring the loading of photobiotin to the photopolymer and for quantification of the measurements , an acoustic sensor technique is used. This system, known as the Love wave device, incorporates a shear horizontal-surface acoustic wave (SH-SAW) overlaid by a thin polymer layer. The polymer layer acts as a waveguide through surface-energy confinement and enhances the sensitivity of mass deposition. Mass adsorbed to the polymer surface changes the phase of the waves during the adsorption process. Finally the SAW technique has used to examine the attachment of biotinylated anti-human IgG antibody. A permanent phase change has again been measured, indicating the functional operation of the photobiotin-avidin complex.
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| Issue date |
2006-09-01 |
| Date available |
2007-03-26 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Physics--Post-graduate theses
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Type of Work--Post-graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/b/8/2/metadata-dlib-2006drakakis.tkl
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| Views |
419 |
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