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Title Μελέτη της ανάπτυξης πράσινων μικροφυκών με χρήση αποβλήτων τυροκομείων ως υπόστρωμα
Alternative Title A study of the growth of green microalgae using cheese-whey waste-waters as a substrate
Author Χατζησεβαστός, Στέργιος
Thesis advisor Γανωτάκης, Δημήτριος
Abstract In the present work, the growth of a photosynthetic microorganism strain of the genus Chlorella, was investigated by using as substrate dairy waste from the primary stage of production. The objective of this work was to investigate the ability of this particular strain in bioremediation applications regarding liquid dairy waste. The target was dual, biomass production and simultaneous decrease of various environmental parameters from the waste-waters. It was observed that microalgae had the ability to grow in dairy waste-waters. However, a limiting factor for cell growth appeared to be the lack of some nutrients, which were either absent in the waste or present in unassimilable form by microbial metabolism. Thus, the enrichment of the waste-waters with nutrients (phosphates, ammonium and trace elements) was also investigated. It was shown that enrichment with nutrients enhanced the production of biomass by the cells. The mixotrophic culture of the cells, with a content of 50% in primary pasteurized whey and with the addition of trace elements led to the highest values of cell concentration and biomass. Chlorella cells were cultured under both heterotrophic and mixotrophic conditions, using as a medium cheese-dairy wastewaters from the primary stage of production. The cultures were allowed to grow for a period of one week. After this period several important parameters were evaluated. These included the evaluation of the content of photosynthetic pigments, maximum photosynthetic efficiency and biomass production. The main conclusion of the present research was that the conditions that favored the growth were directly dependent on the concentration and type of whey, the addition of nutrients and the type of culture (mixotrophic or heterotrophic). It was shown that photosynthetic microorganisms can adapt to different experimental conditions that cause stress, with the ability of simultaneous increase of their biomass. These characteristics provide a perspective for the development of several application regarding food and energy production as well as waste-water management.
Language Greek
Subject Bioremediation
Chlorella sp.
Dairy waste waters (whey)
Απόβλητα τυροκομείου (τυρόγαλα)
Issue date 2021-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
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