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Title Διερεύνηση των συνθηκών ανάπτυξης και παραγωγής υδρογόνου στο μικροφύκος Chlorella sp.
Alternative Title Investigation of growth conditions and hydrogen production by Chlorella sp.
Author Γεωργίτσης, Γεώργιος
Thesis advisor Γανωτάκης, Δημήτριος
Abstract In this thesis, the effect of a series of culture conditions on cell growth, photosynthetic activity and hydrogen production by a photosynthetic microalga of the genus Chlorella, which had been isolated from a eutrophic river in the city of Heraklion, Crete, was investigated. Through the process of photosynthesis, photosynthetic microalgae produce biohydrogen, an environmentally sustainable fuel that has a lower impact on atmospheric pollution. In this context, an investigation was carried out to find the optimal conditions in which Chlorella cells are able to produce higher amounts of hydrogen gas, as well as the effect of these conditions on growth, photosynthetic activity and the biochemical content of the cells. More specifically, the effect of a carbon substrate, temperature, light intensity and agitation of the cultures with an orbital shaker were studied. In addition, a study of hydrogen production in nutrient media with macronutrient deprivation such as sulfur, nitrogen, phosphorus and potassium at different pH was carried out. Also, the biochemical content of the cells in the above conditions was determined. Our results showed that microalgal cells produced the largest amounts of hydrogen when cultured in a nutrient medium where potassium compounds are absent, under slightly acidic conditions. The carbon substrate that led to the highest hydrogen production among other carbon sources was glucose, while medium light intensity regimes, under shaking of the culture produces larger volumes of gas, compared to the other conditions studied. The results of this thesis make it clear that the Chlorella sp. strain has the potential to be used for the production of biofuels with a lower environmental footprint.
Language Greek
Subject Biochemical content
Photosynthetic algae
Sulfur- nitrogen-phosphorus-potassium deprivation
Βιοχημικό περιεχόμενο
Στέρηση θείου - αζώτου - φωσφόρου - καλίου
Συνθήκες ανάπτυξης
Φωτοσυνθετικά μικροφύκη
Issue date 2023-07-27
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/b/d/4/metadata-dlib-1687761192-456504-7494.tkl Bookmark and Share
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