Your browser does not support JavaScript!

Graduate theses

Current Record: 26 of 233

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Title Eosin Y catalyzed synthesis of protein-polymer bioconjugates using different monomers
Alternative Title Σύνθεση βιοσυζυγών πρωτεΐνης-πολυμερούς, καταλυόμενη από Εοσίνη, χρησιμοποιώντας διαφορετικά μονομερή
Author Μαργαριτάκη, Σταυρούλα
Thesis advisor Βελώνια, Καλλιόπη
Abstract The synthesis of protein-polymer conjugates has undoubtedly been a much-discussed topic in the field of polymer science. Protein-polymer conjugates have been synthesized using different approaches with the aim to improve the solubility, stability, biodistribution and half-life of proteins. By taking advantage of the unlimited chemical and structural diversity of polymers, multiple properties of biomacromolecules have been significantly enhanced through polymer bioconjugation. Additionally, the synthesis of amphiphilic protein-polymer conjugates led to biomacromolecules with self-organizing properties which could benefit biotechnological and medical applications such as drug delivery systems, nanoreactors, biosensors, etc. However, since the existing "grafting from" synthetic techniques require laborious, often harsh and disruptive deoxygenation strategies and, "grafting to" approaches mostly result in low yields, studies on applications of proteinpolymer conjugates are still limited. To address these problems and develop a biocompatible and environmentally friendly, synthetic approach, a novel, organocatalyzed, oxygen tolerant, photoinduced, Eosin Y catalyzed methodology was recently developed in the laboratory of Synthetic Biomaterials. Herein, using this novel approach, a range of responsive and hydrophilic monomers were comprehensively screened to identify the optimum reaction conditions leading to quantitative biomacroinitiator consumption. Using this novel, oxygen-tolerant, photoinduced organocatalytic approach we achieved to synthesize well-defined hybrid bioconjugates bearing different polymer moieties were obtained and by carefully altering reaction components and conditions (such as catalyst concentration, monomer loading, pH, cosolvent and salinity). All new protein-polymer conjugates were thoroughly characterized (GPC, NMR, IR, UV). Additionally, the assembling properties of protein-polymer amphiphiles, as imaged through their selfassembled architectures with microscopy, were evaluated.
Language English
Issue date 2022-07-28
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
Notes Τμήμα Επιστήμης και Τεχνολογίας Υλικών, Εργαστήριο Συνθετικών Βιοϋλικών.
Permanent Link https://elocus.lib.uoc.gr//dlib/c/c/3/metadata-dlib-1656674595-984563-1493.tkl Bookmark and Share
Views 276

Digital Documents
No preview available

No permission to view document.
It won't be available until: 2025-07-28