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Title Σύγκριση των βιολογικών χαρακτηριστικώνμεσεγχυματικών/στρωματικών κυττάρων (MSCs)από τη γέλη τουWhartonπριν και μετά την κρυοκατάψυξη του ιστούμε στόχο τηδημιουργία Τράπεζας Ομφαλίου Λώρου
Author Μακρυγιαννάκη, Ειρήνη
Thesis advisor Παπαδάκη, Ελένη
Abstract Mesenchymal Stem / Stromal Cells (MSCs) can be isolated from many tissues in the human body, including Wharton's Jelly tissue (WJ), the most abundant MSCssource inumbilical cord.The continuously increasing use of MSCs in clinical applications makes it necessary to create a bank of MSCs so that they are available and accessible for future use in therapeutic approaches.Everymaintenanceandstoragebankof cell or tissue,should follow acryopreservation protocol that ensures theirefficient and quality storage, so that their biological and functional characteristics remain unchanged. In order to create anumbilical cord bank, we compared umbilicalMSCs derived from WJ beforeand after tissue cryopreservation.Specifically, two groups of MSCs; thePRO-WJMSCs which were isolated and cultured, by the direct method of cell growth and migration to culture surface,and the PT-WJMSCs, MSCs from the same tissue which were obtained by the method of growth / migration after cryopreservationand thawing of Wharton Jelly.During thisstudy,MSCs were isolated from WJ of full-term neonates (n = 8). MSCs in both cases were cultured ex vivo for a total of 9passages (Passage –P) under the same conditions. The immunophenotypic and morphological characteristics of the cells were studied by flow cytometry. In addition, their growthcharacteristics were assessed by evaluating thepopulation doubling time (PDs) and by the methyl-triazolyl-tetrazole (MTT) test during re-cultures.In addition, MSCs senescencewas estimated bymeasuring the relative length of telomeres. Finally, MSCs were evaluatedfor their ability to differentiate into adipocytes and osteocytes. Differentiation was assessed by histochemical staining (Oil Red-O for adipocytes and Alizarin Red/Von Kossa for osteocytes) and by expressionofspecificgenes for adipocytes and osteocytes (PPARG, CEBPA and ALP, RUNX2, OSC, respectively).Analysis of the results showed that both ex vivo cultured MSC populations (PRO-WJMSCs and PT-WJMSCs) exhibitedsimilarmorphological and immunophenotypic characteristics. In addition, they showed no differences in their proliferation capacity. Regarding their potential for differentiation in adipocytes and osteocytes, no significant differences were observed in staining or in the expression of specific genes.Overall, these data so far contribute encouragingly to the creation of anumbilical cord bankas it seems that,thecryopreservation of WJ does not affect the biological and functional 8characteristics of MSCs, providing us with a powerfultoolfor their use in pre-clinical and clinical level.
Language Greek
Issue date 2023
Collection   School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/1/3/f/metadata-dlib-1702378226-268226-16394.tkl Bookmark and Share
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