Post-graduate theses

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Identifier	000460285
Title	Synthesis of multifunctional protein-polymer conjugates using oxygen tolerant approaches
Alternative Title	Σύνθεση λειτουργικών υβριδίων πρωτείνης-πολυμερούς χρησιμοποιώντας ανεκτικές στο οξυγόνο μεθόδους πολυμερισμού
Author	Μπούση, Γιονίντα
Thesis advisor	Βελώνια, Καλλιόπη
Reviewer	Ρεμεδιάκης, Ιωάννης Αρματάς, Γεράσιμος
Abstract	Protein-polymer conjugates hybrid materials that have the potential to exhibit properties of both the biomolecule and the synthetic polymer. As such, they are expected to find application in a variety of sectors such as medicine, biotechnology, diagnostics among others. Focus in this Thesis was the synthesis of multifunctional protein-polymer conjugates using different oxygen tolerant techniques. An oxygen-tolerant, aqueous copper-mediated polymerization approach, and an alternative, ligand-free methodology were used to synthesize protein-polymer conjugates and triblock bioconjugates in high yields. The effect of hydrophobic/hydrophilic ratio, chemical composition and temperature on the self-assembly of the biohybrid products was evaluated. Finally, a significant contribution into the development of a new, oxygen-tolerant methodology for the synthesis of protein-polymer conjugates via organocatalysis is presented in this Thesis. This greener and more sustainable approach was used to synthesize enzyme bioconjugates and evaluate their catalytic activity. Size Exclusion Chromatography SEC), Native Polyacrylamide Gel Electrophoresis (PAGE), Nuclear Magnetic Resonance Spectroscopy (NMR), Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetric analysis (TGA) and Field Emission Scanning Electron Microscopy (FE-SEM) were used characterize the products.
Language	English
Subject	Bioconjugate
	Hybrid materials
	Self assembly
	Αυτοοργάνωση
	Βιοσύζευξη
	Υβρίδια Πρωτεϊνης-πολυμερούς
	Υβριδικά υλικά
Issue date	2023-11-29
Collection	School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/2/d/5/metadata-dlib-1699603335-928469-8695.tkl
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