Post-graduate theses
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Identifier |
000460285 |
Title |
Synthesis of multifunctional protein-polymer conjugates using oxygen tolerant approaches |
Alternative Title |
Σύνθεση λειτουργικών υβριδίων πρωτείνης-πολυμερούς χρησιμοποιώντας ανεκτικές στο οξυγόνο μεθόδους πολυμερισμού |
Author
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Μπούση, Γιονίντα
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Thesis advisor
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Βελώνια, Καλλιόπη
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Reviewer
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Ρεμεδιάκης, Ιωάννης
Αρματάς, Γεράσιμος
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Abstract |
Protein-polymer conjugates hybrid materials that have the potential to exhibit properties of both the biomolecule and the synthetic polymer. As such, they are expected to find application in a variety of sectors such as medicine, biotechnology, diagnostics among others.
Focus in this Thesis was the synthesis of multifunctional protein-polymer conjugates using different oxygen tolerant techniques. An oxygen-tolerant, aqueous copper-mediated polymerization approach, and an alternative, ligand-free methodology were used to synthesize protein-polymer conjugates and triblock bioconjugates in high yields. The effect of hydrophobic/hydrophilic ratio, chemical composition and temperature on the self-assembly of the biohybrid products was evaluated. Finally, a significant contribution into the development of a new, oxygen-tolerant methodology for the synthesis of protein-polymer conjugates via organocatalysis is presented in this Thesis. This greener and more sustainable approach was used to synthesize enzyme bioconjugates and evaluate their catalytic activity.
Size Exclusion Chromatography SEC), Native Polyacrylamide Gel Electrophoresis (PAGE), Nuclear Magnetic Resonance Spectroscopy (NMR), Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetric analysis (TGA) and Field Emission Scanning Electron Microscopy (FE-SEM) were used characterize the products.
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Language |
English |
Subject |
Bioconjugate |
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Hybrid materials |
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Self assembly |
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Αυτοοργάνωση |
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Βιοσύζευξη |
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Υβρίδια Πρωτεϊνης-πολυμερούς |
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Υβριδικά υλικά |
Issue date |
2023-11-29 |
Collection
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School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/2/d/5/metadata-dlib-1699603335-928469-8695.tkl
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Views |
1311 |
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